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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/08/2019 |
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Data da última atualização: |
12/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVARES, R. C.; STONEHOUSE, R.; SOUZA, T. L. P. O.; MELO, P. G. S.; MIKLAS, P. N.; BETT, K. E.; MELO, L. C.; RODRIGUES, L. A.; SOUZA, L. L.; PEREIRA, H. S. |
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Afiliação: |
RENATA C. ALVARES, UFG; ROBERT STONEHOUSE, UNIVERSITY OF SASKATCHEWAN, Canada; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; PATRICIA G. S. MELO, UFG; PHILLIP N. MIKLAS, USDA, Prosser-WA; KIRSTIN E. BETT, UNIVERSITY OF SASKATCHEWAN, Canada; LEONARDO CUNHA MELO, CNPAF; LUANA ALVES RODRIGUES, CNPAF; LORENA L. SOUZA, UFG; HELTON SANTOS PEREIRA, CNPAF. |
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Título: |
Generation and validation of genetic markers for the selection of carioca dry bean genotypes with the slow-darkening seed coat trait. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Euphytica, v. 215, n. 8, Aug. 2019. |
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ISSN: |
1573-5060 |
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DOI: |
10.1007/s10681-019-2461-y |
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Idioma: |
Inglês |
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Conteúdo: |
Slow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepérola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd-1158 and PVM02TC116 and with a TaqMan marker designed for the single-nucleotide polymorphism (SNP) PvbHLHp12804. A KASP marker was also designed for the PvbHLHp12804 marker for testing on advanced carioca lines developed by the University of Saskatchewan. In the carioca lines developed by Embrapa, PVM02TC116 proved unsuitable for marker-assisted selection (MAS). Both the Pvsd-1158 and PvbHLHp12804 markers were found to be tightly linked to the gene responsible for the SD trait, with genetic distances calculated at 2.8 cM for Pvsd-1158 and 2.0 and 3.1 cM for PvbbHLHp12804, respectively. These markers presented more than 97% of selection efficiency. The genotypic scoring using the PvbHLHp12804 KASP marker was perfectly correlated with the phenotype in all lines of the University of Saskatchewan. The results of this study validates the use of Pvsd-1158 as a gel-based marker for SD in carioca beans. The new fluorescence-based SNP PvbHLHp12804 markers exhibited very tight linkage to SD in carioca and pinto bean lines. These markers will be ideal for MAS for the SD trait in these market classes. MenosSlow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepérola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd-1158 and PVM02TC116 and with a TaqMan marker designed for the single-nucleotide polymorphism (SNP) PvbHLHp12804. A KASP marker was also designed for the PvbHLHp12804 marker for testing on advanced carioca lines developed by the University of Saskatchewan. In the carioca lines developed by Embrapa, PVM02TC116 proved unsuitable for marker-assisted selection (MAS). Both the Pvsd-1158 and PvbHLHp12804 markers were found to be tightly linked to the gene responsible for the SD trait, with genetic distances calculated at 2.8 cM for Pvsd-1158 and 2.0 and 3.1 cM for PvbbHLHp12804, respectively. These markers presented more than 97% of selection efficiency. The genotypic scoring using the PvbHLHp12804 KASP marker was perfectly correlated with the phenotype in all lines of the University of Saskatchewan. The results of this study validates the use of Pvsd-1158... Mostrar Tudo |
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Palavras-Chave: |
SNP; SSR. |
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Thesagro: |
Feijão; Genótipo; Marcador Molecular; Melhoramento Genético Vegetal; Phaseolus Vulgaris. |
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Thesaurus Nal: |
Beans; Marker-assisted selection. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02782naa a2200361 a 4500
001 2111158
005 2020-02-12
008 2019 bl uuuu u00u1 u #d
022 $a1573-5060
024 7 $a10.1007/s10681-019-2461-y$2DOI
100 1 $aALVARES, R. C.
245 $aGeneration and validation of genetic markers for the selection of carioca dry bean genotypes with the slow-darkening seed coat trait.$h[electronic resource]
260 $c2019
520 $aSlow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepérola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd-1158 and PVM02TC116 and with a TaqMan marker designed for the single-nucleotide polymorphism (SNP) PvbHLHp12804. A KASP marker was also designed for the PvbHLHp12804 marker for testing on advanced carioca lines developed by the University of Saskatchewan. In the carioca lines developed by Embrapa, PVM02TC116 proved unsuitable for marker-assisted selection (MAS). Both the Pvsd-1158 and PvbHLHp12804 markers were found to be tightly linked to the gene responsible for the SD trait, with genetic distances calculated at 2.8 cM for Pvsd-1158 and 2.0 and 3.1 cM for PvbbHLHp12804, respectively. These markers presented more than 97% of selection efficiency. The genotypic scoring using the PvbHLHp12804 KASP marker was perfectly correlated with the phenotype in all lines of the University of Saskatchewan. The results of this study validates the use of Pvsd-1158 as a gel-based marker for SD in carioca beans. The new fluorescence-based SNP PvbHLHp12804 markers exhibited very tight linkage to SD in carioca and pinto bean lines. These markers will be ideal for MAS for the SD trait in these market classes.
650 $aBeans
650 $aMarker-assisted selection
650 $aFeijão
650 $aGenótipo
650 $aMarcador Molecular
650 $aMelhoramento Genético Vegetal
650 $aPhaseolus Vulgaris
653 $aSNP
653 $aSSR
700 1 $aSTONEHOUSE, R.
700 1 $aSOUZA, T. L. P. O.
700 1 $aMELO, P. G. S.
700 1 $aMIKLAS, P. N.
700 1 $aBETT, K. E.
700 1 $aMELO, L. C.
700 1 $aRODRIGUES, L. A.
700 1 $aSOUZA, L. L.
700 1 $aPEREIRA, H. S.
773 $tEuphytica$gv. 215, n. 8, Aug. 2019.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| 1. |  | PAULA, L. E. de R. e; TRUGILHO, P. F.; ARANTES, M. D. C.; REZENDE, R. N. Avaliação tecnológica do carvão vegetal do coco babaçu. In: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. (Embrapa Florestas. Documentos, 178). Seção: Sustentabilidade Ambiental, Social e Econômica.| Biblioteca(s): Embrapa Florestas. |
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