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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
20/12/2018 |
Data da última atualização: |
20/12/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARTI, E. C. F. B.; RODRIGUES, R. A.; DUARTE, L. F. C.; BACANELLI, G. M.; LILENBAUM, W.; ARAUJO, F. R. |
Afiliação: |
Universidade Federal do Mato Grosso do Sul - UFMS; Universidade Federal do Mato Grosso do Sul - UFMS; Universidade Federal do Mato Grosso do Sul - UFMS; Universidade Federal do Mato Grosso do Sul - UFMS; Laboratório de Bacteriologia Veterinária/Universidade Federal Fluminense; FLABIO RIBEIRO DE ARAUJO, CNPGC. |
Título: |
Detection of Potentially Pathogenic Non-Tuberculous Mycobacteria in Artisanal Coalho Cheese from the State of Paraiba, Northeast Brazil. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Mycobacterial Diseases, v. 8, n. 3, 2018 |
Idioma: |
Inglês |
Conteúdo: |
The artisanal Coalho cheese is one of the most consumed dairy products in some regions of Brazil, especially in the Northeastern. Because it is minimally ripened, it should be produced using heat-treated milk, however, its manufacture commonly uses raw milk. Reports of contamination of Coalho cheese with pathogenic bacteria are increasing, including Mycobacterium spp. Non-tuberculous mycobacteria (NTM) are emerging pathogens that cause infections in humans. This study describes the identification of viable NTM in artisanal Coalho cheese in the State of Paraíba, Northeastern, Brazil. On hundred samples of artisanal Coalho cheese, produced without sanitary inspection, were collected at street markets in the regions of Borborema, Agreste and Mata, and cultured in Stonebrink medium. Isolates were analyzed by PCR for hsp65 of Mycobacterium spp. and the DNA amplicons were sequenced. The resulting sequences were submitted to identity search by Blastn (NCBI). Colonies were isolated in 5/100 (5%) samples of Coalho cheese, which were positive in the PCR for hsp65. The resulting DNA consensus sequences showed similarity to hsp65 from Mycobacterium fortuitum (100% identity), Mycobacterium novocastrense (99% identity), Bifidobacterium crudilactis (98% identity), Kocuria rhizophila (98% identity) and Kocuria palustris (91% identity).The consumption of artisanal Coalho cheese from street markets represents a risk for human health, due to the possibility of transmission of non-tuberculous mycobacteria and other actinobacteria potentially pathogenic. This study reinforces the need for the establishment of public policies to prevent the commercialization of Coalho cheese, produced without sanitary inspection. MenosThe artisanal Coalho cheese is one of the most consumed dairy products in some regions of Brazil, especially in the Northeastern. Because it is minimally ripened, it should be produced using heat-treated milk, however, its manufacture commonly uses raw milk. Reports of contamination of Coalho cheese with pathogenic bacteria are increasing, including Mycobacterium spp. Non-tuberculous mycobacteria (NTM) are emerging pathogens that cause infections in humans. This study describes the identification of viable NTM in artisanal Coalho cheese in the State of Paraíba, Northeastern, Brazil. On hundred samples of artisanal Coalho cheese, produced without sanitary inspection, were collected at street markets in the regions of Borborema, Agreste and Mata, and cultured in Stonebrink medium. Isolates were analyzed by PCR for hsp65 of Mycobacterium spp. and the DNA amplicons were sequenced. The resulting sequences were submitted to identity search by Blastn (NCBI). Colonies were isolated in 5/100 (5%) samples of Coalho cheese, which were positive in the PCR for hsp65. The resulting DNA consensus sequences showed similarity to hsp65 from Mycobacterium fortuitum (100% identity), Mycobacterium novocastrense (99% identity), Bifidobacterium crudilactis (98% identity), Kocuria rhizophila (98% identity) and Kocuria palustris (91% identity).The consumption of artisanal Coalho cheese from street markets represents a risk for human health, due to the possibility of transmission of non-tuberculous m... Mostrar Tudo |
Palavras-Chave: |
Coalho cheese; Microbiological culture. |
Thesaurus Nal: |
Kocuria rhizophila; Mycobacterium fortuitum; Mycobacterium novocastrense. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02497naa a2200241 a 4500 001 2102165 005 2018-12-20 008 2018 bl uuuu u00u1 u #d 100 1 $aSARTI, E. C. F. B. 245 $aDetection of Potentially Pathogenic Non-Tuberculous Mycobacteria in Artisanal Coalho Cheese from the State of Paraiba, Northeast Brazil.$h[electronic resource] 260 $c2018 520 $aThe artisanal Coalho cheese is one of the most consumed dairy products in some regions of Brazil, especially in the Northeastern. Because it is minimally ripened, it should be produced using heat-treated milk, however, its manufacture commonly uses raw milk. Reports of contamination of Coalho cheese with pathogenic bacteria are increasing, including Mycobacterium spp. Non-tuberculous mycobacteria (NTM) are emerging pathogens that cause infections in humans. This study describes the identification of viable NTM in artisanal Coalho cheese in the State of Paraíba, Northeastern, Brazil. On hundred samples of artisanal Coalho cheese, produced without sanitary inspection, were collected at street markets in the regions of Borborema, Agreste and Mata, and cultured in Stonebrink medium. Isolates were analyzed by PCR for hsp65 of Mycobacterium spp. and the DNA amplicons were sequenced. The resulting sequences were submitted to identity search by Blastn (NCBI). Colonies were isolated in 5/100 (5%) samples of Coalho cheese, which were positive in the PCR for hsp65. The resulting DNA consensus sequences showed similarity to hsp65 from Mycobacterium fortuitum (100% identity), Mycobacterium novocastrense (99% identity), Bifidobacterium crudilactis (98% identity), Kocuria rhizophila (98% identity) and Kocuria palustris (91% identity).The consumption of artisanal Coalho cheese from street markets represents a risk for human health, due to the possibility of transmission of non-tuberculous mycobacteria and other actinobacteria potentially pathogenic. This study reinforces the need for the establishment of public policies to prevent the commercialization of Coalho cheese, produced without sanitary inspection. 650 $aKocuria rhizophila 650 $aMycobacterium fortuitum 650 $aMycobacterium novocastrense 653 $aCoalho cheese 653 $aMicrobiological culture 700 1 $aRODRIGUES, R. A. 700 1 $aDUARTE, L. F. C. 700 1 $aBACANELLI, G. M. 700 1 $aLILENBAUM, W. 700 1 $aARAUJO, F. R. 773 $tMycobacterial Diseases$gv. 8, n. 3, 2018
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Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
23/05/2006 |
Data da última atualização: |
13/06/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
BRESEGHELLO, F.; SOUSA, N. R. G. de; BARROS, L. G. de. |
Afiliação: |
FLAVIO BRESEGHELLO, CNPAF; NARA REGINA GERVINI DE SOUSA; LUIZ GONZAGA DE BARROS. |
Título: |
Melhoramento genético de arroz no Estado de Mato Grosso, no período de 1981 a 1997. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
In: REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 6., 1998, Goiânia. Perspectivas para a cultura dos arroz nos ecossistemas de várzeas e terras altas: resumos expandidos. Goiânia: Embrapa-CNPAF, 1998. |
Volume: |
v. 1. |
Páginas: |
p. 268-271. |
Série: |
(Embrapa-CNPAF. Documentos, 85). |
ISSN: |
1516-7518 |
Idioma: |
Português |
Palavras-Chave: |
Mato Grosso. |
Thesagro: |
Arroz; Melhoramento genético vegetal; Oryza sativa. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162912/1/CNPAF-1998-p268.pdf
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Marc: |
LEADER 00802nam a2200205 a 4500 001 1197984 005 2023-06-13 008 1998 bl uuuu u00u1 u #d 022 $a1516-7518 100 1 $aBRESEGHELLO, F. 245 $aMelhoramento genético de arroz no Estado de Mato Grosso, no período de 1981 a 1997.$h[electronic resource] 260 $aIn: REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 6., 1998, Goiânia. Perspectivas para a cultura dos arroz nos ecossistemas de várzeas e terras altas: resumos expandidos. Goiânia: Embrapa-CNPAF$c1998 300 $ap. 268-271. v. 1. 490 $a(Embrapa-CNPAF. Documentos, 85).$vv. 1. 650 $aArroz 650 $aMelhoramento genético vegetal 650 $aOryza sativa 653 $aMato Grosso 700 1 $aSOUSA, N. R. G. de 700 1 $aBARROS, L. G. de
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Embrapa Arroz e Feijão (CNPAF) |
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