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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
29/11/2018 |
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Data da última atualização: |
25/06/2019 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. |
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Afiliação: |
BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Setaria viridis as a model plant for functional genomic studies in C4 Crops. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). |
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Páginas: |
p. 49-64 |
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Idioma: |
Inglês |
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Conteúdo: |
Setaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops. |
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Palavras-Chave: |
Embryogenic callus; Floral dip; Transformation. |
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Thesaurus Nal: |
Agrobacterium; Grasses. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01445naa a2200229 a 4500
001 2100359
005 2019-06-25
008 2019 bl uuuu u00u1 u #d
100 1 $aMARTINS, P. K.
245 $aSetaria viridis as a model plant for functional genomic studies in C4 Crops.$h[electronic resource]
260 $c2019
300 $ap. 49-64
520 $aSetaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops.
650 $aAgrobacterium
650 $aGrasses
653 $aEmbryogenic callus
653 $aFloral dip
653 $aTransformation
700 1 $aDIAS, B. B. A.
700 1 $aKOBAYASHI, A. K.
700 1 $aMOLINARI, H. B. C.
773 $tIn: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology$gv. 1864).
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
21/06/2018 |
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Data da última atualização: |
25/02/2019 |
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Autoria: |
FORTE, C. T.; GALON, L.; BEUTLER, A. N.; BASSO, F. J. M.; NONEMACHER, F.; REICHERT JÚNIOR, F. W.; PERIN, G. F.; TIRONI, S. P. |
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Afiliação: |
Cesar Tiago Forte, Universidade Federal da Fronteira Sul/Campus Erechim; Leandro Galon, Universidade Federal da Fronteira Sul/Campus Erechim; Amauri Nelson Beutler, Universidade Federal do Pampa/Campus Itaqui; Felipe José Menin Basso, Universidade Federal da Fronteira Sul/Campus Erechim; Felipe Nonemacheri, Universidade Federal da Fronteira Sul/Campus Erechim; Francisco Wilson Reichert Júnior, Universidade Federal da Fronteira Sul/Campus Erechim; Gismael Francisco Perin, Universidade Federal da Fronteira Sul/Campus Erechim; Siumar Pedro Tironi, Universidade Federal da Fronteira Sul/Campus Chapecó. |
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Título: |
Soil management systems and their effect on the weed seed bank. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 4, p. 435-442, abr. 2018. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Sistemas de manejo do solo e sua influência no banco de sementes de plantas daninhas. |
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Conteúdo: |
The objective of this work was to evaluate the density and composition of the soil weed seed bank when bean, corn, and soybean are cultivated in the no-tillage system (NTS) in rotation with winter cover crop species and in the conventional tillage system (CTS). The experiment was installed in a complete randomized block design with three replicates. The evaluation of the seed bank was performed on soil samples (0?10 and 10?20 cm) in four points of each experimental unit, at 15, 30, 60, and 90 days of cultivation. Bean, corn, and soybean crops were sown in the NTS with different soil cover crops in rotation, as well as in the CTS. The NTS provided a more dense and abundant soil seed bank of the species Gnaphalium spicatum and Oxalis corniculata when corn, soybean, and bean were cultivated. The species Lolium multiflorum showed lower density and less seeds in the soil seed bank when the NTS was adopted. The use of the winter cover crops black oat and cow vetch, cultivated individually or in consortium, resulted in a lower density of weed species, especially of L. multiflorum. The NTS provides a lower density of weed species in the soil seed bank than the CTS. |
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Palavras-Chave: |
Conventional tillage system; Sistema plantio convencional; Sistema plantio direto. |
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Thesagro: |
Avena Strigosa; Raphanus Sativus; Vicia Sativa. |
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Thesaurus NAL: |
No-tillage. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178849/1/Soil-management-systems-and-their-effect.pdf
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Marc: |
LEADER 02162naa a2200301 a 4500
001 2092650
005 2019-02-25
008 2018 bl uuuu u00u1 u #d
100 1 $aFORTE, C. T.
245 $aSoil management systems and their effect on the weed seed bank.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Sistemas de manejo do solo e sua influência no banco de sementes de plantas daninhas.
520 $aThe objective of this work was to evaluate the density and composition of the soil weed seed bank when bean, corn, and soybean are cultivated in the no-tillage system (NTS) in rotation with winter cover crop species and in the conventional tillage system (CTS). The experiment was installed in a complete randomized block design with three replicates. The evaluation of the seed bank was performed on soil samples (0?10 and 10?20 cm) in four points of each experimental unit, at 15, 30, 60, and 90 days of cultivation. Bean, corn, and soybean crops were sown in the NTS with different soil cover crops in rotation, as well as in the CTS. The NTS provided a more dense and abundant soil seed bank of the species Gnaphalium spicatum and Oxalis corniculata when corn, soybean, and bean were cultivated. The species Lolium multiflorum showed lower density and less seeds in the soil seed bank when the NTS was adopted. The use of the winter cover crops black oat and cow vetch, cultivated individually or in consortium, resulted in a lower density of weed species, especially of L. multiflorum. The NTS provides a lower density of weed species in the soil seed bank than the CTS.
650 $aNo-tillage
650 $aAvena Strigosa
650 $aRaphanus Sativus
650 $aVicia Sativa
653 $aConventional tillage system
653 $aSistema plantio convencional
653 $aSistema plantio direto
700 1 $aGALON, L.
700 1 $aBEUTLER, A. N.
700 1 $aBASSO, F. J. M.
700 1 $aNONEMACHER, F.
700 1 $aREICHERT JÚNIOR, F. W.
700 1 $aPERIN, G. F.
700 1 $aTIRONI, S. P.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 53, n. 4, p. 435-442, abr. 2018.
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