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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Suínos e Aves. |
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Data corrente: |
13/11/2018 |
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Data da última atualização: |
03/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OKINO, C. H.; IBELLI, A. M. G.; BRENTANO, L.; ZANELLA, R.; MARQUES, M. G. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; LIANA BRENTANO, CNPSA; Ricardo Zanella, Universidade de Passo Fundo; MARIANA GROKE MARQUES, CNPSA. |
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Título: |
Evaluation of candidate reference genes for investigation of the uterine tissue and corpus luteum of pigs on day 6 after artificial insemination. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 198, p. 202-209, 2018. |
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DOI: |
10.1016/j.anireprosci.2018.09.020 |
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Idioma: |
Inglês |
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Conteúdo: |
Housekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative abundance of mRNA and that this gene could be used as a reference for corpora lutea analyses of mRNA MenosHousekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative a... Mostrar Tudo |
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Palavras-Chave: |
GAPDH; Housekeeping; TOP2B. |
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Thesagro: |
Inseminação; Reprodução Animal; Suíno; Útero. |
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Thesaurus Nal: |
Gene expression; Sows; Ubiquitin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02483naa a2200301 a 4500
001 2099280
005 2019-01-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.anireprosci.2018.09.020$2DOI
100 1 $aOKINO, C. H.
245 $aEvaluation of candidate reference genes for investigation of the uterine tissue and corpus luteum of pigs on day 6 after artificial insemination.$h[electronic resource]
260 $c2018
520 $aHousekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative abundance of mRNA and that this gene could be used as a reference for corpora lutea analyses of mRNA
650 $aGene expression
650 $aSows
650 $aUbiquitin
650 $aInseminação
650 $aReprodução Animal
650 $aSuíno
650 $aÚtero
653 $aGAPDH
653 $aHousekeeping
653 $aTOP2B
700 1 $aIBELLI, A. M. G.
700 1 $aBRENTANO, L.
700 1 $aZANELLA, R.
700 1 $aMARQUES, M. G.
773 $tAnimal Reproduction Science$gv. 198, p. 202-209, 2018.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 3. |  | SILVA, V. T.; SILVA, G. T.; LUCAS, N. F.; SCIVITTARO, W. B.; SOUSA, R. O. DE. Potencial de aquecimento global de cultivares de arroz sob irrigação por inundação contínua e intermitente. In: SEMANA INTEGRADA UFPEL, 6.; CONGRESSO DE INICIAÇÃO CIENTÍFICA, 19., 2020, Pelotas. [Anais... Pelotas: UFPel, 2020.]. 4 p. CIC.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | CAMARGO, R. R. de; SILVA, W. R. da; SILVA, V. T. da; SCARIOTTO, S.; RASEIRA, M. do C. B. Conservação de pólen de pessegueiros sob diferentes temperaturas. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 22.; ENCONTRO DE PÓS-GRADUAÇÃO, 15., 2013, Pelotas. [Anais.]. Pelotas: UFPel, 2013.| Tipo: Artigo em Anais de Congresso |
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