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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
23/01/2017 |
Data da última atualização: |
23/01/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CALVI, G. P.; AUD, F. F.; FERRAZ, I. D. K.; PRITCHARD, H. W.; KRANNER, I. |
Afiliação: |
G. P. CALVI, INPA; FABIANA FERRAZ AUD, CNPMF; I. D. K. FERRAZ, INPA; H. W. PRITCHARD, Royal Botanic Gardens; I. KRANNER, Royal Botanic Gardens. |
Título: |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Plant Biology, n. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017. |
ISSN: |
1435-8603 |
Idioma: |
Inglês |
Conteúdo: |
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. MenosThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towar... Mostrar Tudo |
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Semente. |
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Marc: |
LEADER 02347naa a2200193 a 4500 001 2061470 005 2017-01-23 008 2017 bl uuuu u00u1 u #d 022 $a1435-8603 100 1 $aCALVI, G. P. 245 $aAnalyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination.$h[electronic resource] 260 $c2017 520 $aThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. 650 $aSemente 700 1 $aAUD, F. F. 700 1 $aFERRAZ, I. D. K. 700 1 $aPRITCHARD, H. W. 700 1 $aKRANNER, I. 773 $tPlant Biology$gn. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
25/11/2022 |
Data da última atualização: |
25/11/2022 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SAMPAIO, J. A. G.; GIMENES, M. A.; SILVA, F. A. da; BENITO, N. P.; BONATO, G.; SALOMAO, A. N.; SALES, P. V. R.; RODRIGUES, N. C. da C.; CARVALHO, R. V. de; PAIVA, S. R. |
Afiliação: |
MARCOS APARECIDO GIMENES, Cenargen; FERNANDA ALVARES DA SILVA, Cenargen; NORTON POLO BENITO, Cenargen; GLADYES BONATO, Cenargen; ANTONIETA NASSIF SALOMAO, Cenargen; POLIANA VIEIRA RODRIGUES SALES, SUSEC; NAIARA CREAO DA COSTA, SUSEC; ROBERTO VIEIRA DE CARVALHO, SUSEC; SAMUEL REZENDE PAIVA, Cenargen. |
Título: |
Intercâmbio de germoplasma vegetal na Embrapa de 2019 a 2021. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
In: Congresso Brasileiro de Recursos Genéticos, 7., 2022. Multifuncionalidade e Qualidade de Vida: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2022. |
Idioma: |
Português |
Notas: |
Poster. Na publicação: Naiara Creão da Costa. |
Palavras-Chave: |
Intercâmbio; Sistema Nacional de Pesquisa Agrícola. |
Thesagro: |
Quarentena. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00909nam a2200253 a 4500 001 2148784 005 2022-11-25 008 2022 bl uuuu u00u1 u #d 100 1 $aSAMPAIO, J. A. G. 245 $aIntercâmbio de germoplasma vegetal na Embrapa de 2019 a 2021.$h[electronic resource] 260 $aIn: Congresso Brasileiro de Recursos Genéticos, 7., 2022. Multifuncionalidade e Qualidade de Vida: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2022 500 $aPoster. Na publicação: Naiara Creão da Costa. 650 $aQuarentena 653 $aIntercâmbio 653 $aSistema Nacional de Pesquisa Agrícola 700 1 $aGIMENES, M. A. 700 1 $aSILVA, F. A. da 700 1 $aBENITO, N. P. 700 1 $aBONATO, G. 700 1 $aSALOMAO, A. N. 700 1 $aSALES, P. V. R. 700 1 $aRODRIGUES, N. C. da C. 700 1 $aCARVALHO, R. V. de 700 1 $aPAIVA, S. R.
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