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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
12/09/2016 |
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Data da última atualização: |
13/04/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SA, F. P. de; LEDO, A. da S.; AMORIM, J. A. E.; MUNIZ, A. V. C. da S.; PASQUAL, M. |
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Afiliação: |
FRANCIELEN PAOLA DE SA; ANA DA SILVA LEDO, CPATC; JULIE ANNE ESPINDOLA AMORIM; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; MOACIR PASCOAL. |
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Título: |
In vitro propagation and acclimatization of genipapo accessions. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, Lavras, MG, v. 40, n. 2, p. 155-163, mar./abr. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT® may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed. MenosGenipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato ... Mostrar Tudo |
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Palavras-Chave: |
Genética de planta; Genipapo. |
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Thesagro: |
Fruta tropical; Jenipapo. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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| 1. |  | BEZERRA, K. R. A.; CAMPANHA, M. M.; MERA, R. D. M.; BEZERRA, F. G. S.; MAYORGA, M. I. de O.; GUIMARÃES, V. P. Valoração econômica dos serviços ambientais em um sistema agrossilvipastoril no bioma caatinga. In: CONGRESSO BRASILEIRO DE AGROECOLOGIA, 7., 2011, Fortaleza. Ética na ciência: agroecologia como paradigma para o desenvolvimento rural. Fortaleza: Universidade Federal do Ceará, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Milho e Sorgo. |
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