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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
03/08/2016 |
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Data da última atualização: |
07/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIGLIOTI, A.; OLIVEIRA, H. N.; SANTANA, C. H.; IBELLI, A. M. G.; NÉO, T. A.; BILHASSI, T. B.; RABELO, M. D.; MACHADO, R. Z.; BRITO, L. G.; OLIVEIRA, M. C. de S. |
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Afiliação: |
Unesp (Jaboticabal); Unesp (Jaboticabal); Unesp (Jaboticabal); Unesp (Jaboticabal); UFSCAR; Unesp (Jaboticabal); MARCIO DIAS RABELO, CPPSE; Unesp (Jaboticabal); LUCIANA GATTO BRITO, CPAF-Rondonia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 7, p. 657-66, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal. MenosThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus a... Mostrar Tudo |
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Palavras-Chave: |
QPCR; R microplus; Resistance. |
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Thesaurus Nal: |
babesiosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03159naa a2200277 a 4500
001 2050164
005 2022-03-07
008 2016 bl uuuu u00u1 u #d
100 1 $aGIGLIOTI, A.
245 $aBabesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of São Paulo state, Brazil.$h[electronic resource]
260 $c2016
520 $aThe levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P<0.05) between the NC of the two Babesia species, B. bovis 1.49±0.07 vs. B. bigemina 0.82±0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal.
650 $ababesiosis
653 $aQPCR
653 $aR microplus
653 $aResistance
700 1 $aOLIVEIRA, H. N.
700 1 $aSANTANA, C. H.
700 1 $aIBELLI, A. M. G.
700 1 $aNÉO, T. A.
700 1 $aBILHASSI, T. B.
700 1 $aRABELO, M. D.
700 1 $aMACHADO, R. Z.
700 1 $aBRITO, L. G.
700 1 $aOLIVEIRA, M. C. de S.
773 $tTicks and Tick-borne Diseases$gv. 7, p. 657-66, 2016.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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| Registros recuperados : 15 | |
| 7. |  | SILVEIRA, M. R. S. da; ALVES, R. E.; MOURA, C. F. H.; FIGUEIREDO, R. W. de; FARIAS, J. M. de. Caracterização de frutos de genótipos de puçazeiro 'Coroa de Frade' oriundos da vegetação litorânea do Ceará. In: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA, 2., 2007, Viçosa, MG. Frutas, hortaliças e flores: palestras e resumos. Viçosa: UFV, 2007. p. 361| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 8. | | MACIEL, V. T.; GOMES FILHO, E.; ALVES, R. E.; FARIAS, J. M. de; SOUZA, H. U. de. Caracterização física dos frutos de seis cultivares de coqueiro anão em diferentes estádios de desenvolvimento. Agrária - Revista Brasileira de Ciências Agrárias, Recife, v. 4, n. 4, p. 395-398, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 9. | | MACIEL, V. T.; GOMES FILHO, E.; ALVES, R. E.; FARIAS, J. M. de; SOUSA, H. U. de. Caracterização física dos frutos de seis cultivares de coqueiro anão em diferentes estágios de desenvolvimento. Revista Brasileira de Ciências Agrárias, Recife, v. 4, n. 4, p. 395-398, out./dez. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Acre. |
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| 10. | | MACIEL, V. T.; GOMES FILHO, E.; ALVES, R. E.; FARIAS, J. M. de; MOURA, C. F. H.; SOUSA, H. U. de. Atividade de enzimas anti-oxidativas na água de cocos em diferentes estádios de desenvolvimento. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. 4 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 11. | | MALAQUIAS, J. V.; GAMA, L. C.; PELUFE, M. S.; ARAUJO, S. da L. S.; MIRANDA, Z. de J. G.; FARIAS, J. M. de S.; CRUZ, C. V. Avaliação da comunicação interna: novas perspectivas sobre os veículos de comunicação da Embrapa Cerrados. Planaltina, DF: Embrapa Cerrados, 2012. 56 p. (Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 306).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Cerrados. |
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| 12. | | SANTOS FILHA, M. E. C. dos; ALVES, R. E.; SOUSA, H. U. de; MACIEL, V. T.; FARIAS, J. M. de; RUFINO, M. do S. M. Características físicas de frutos de seis cultivares de coqueiro anão. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 3., 2005, Gramado. Anais... Passo Fundo: Embrapa Trigo: SBMP, 2005. 1 CD-ROM. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 13. | | SILVA, D. L. V. da; ALVES, R. E.; FIGUEIREDO, R. W. de; MACIEL, V. T.; FARIAS, J. M. de; AQUINO, A. R. L. de. Características físicas, físico-químicas e sensoriais da água de frutos de coqueiro anão verde oriundo de produção convencional e orgânica. Ciência e Agrotecnologia, Lavras, v. 33, n. 4, p. 1079-1084, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 14. | | FONTENELE. M. A.; RIBEIRO, J. F.; FARIAS, J. M. de; SOARES, D. J.; FIGUEIREDO, R. W. de; MAIA, G. A.; ALVES, R. E. Conservação pós-colheita de bacuri (Platonia Insignis Mart) colhido na planta, armazenado sob condições ambiente e atmosfera modificada, oriundos do Estado do Maranhão. In: CONGRESSO LATINO AMERICANO DE ANALISTAS DE ALIMENTOS, 15., 2007, Fortaleza-CE. Anais... Fortaleza: SBAAL, 2007. 4 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 15. | | SANTOS FILHA, M. E. C. dos; ALVES, R. E.; SOUSA, H. U. de; MACIEL, V. T.; FARIAS, J. M. de; RUFINO, M. do S. M. Qualidade da água de frutos de seis cultivares de coqueiro anão para consumo in natura e/ou industrialização. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 3., 2005, Gramado. Anais... Passo Fundo: Embrapa Trigo: SBMP, 2005. 1 CD-ROM. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 15 | |
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