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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
03/03/2017 |
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Data da última atualização: |
03/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, M. I. G.; SANT'ANA, A. E. G.; VASCONCELOS, F. M. T.; SILVA, W. L.; LIMA, L. M. de; CARVALHO, R.; MELO FILHO, P. A.; SANTOS, R. C. dos. |
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Afiliação: |
MARIA ISABEL G. MARTINS, RENORBIO/UFRPE; ANTONIO EUZÉBIO G. SANT'ANA, UFAL; FELIPE MATHEUS T. VASCONCELOS, UFRPE; WBYRATAN L. SILVA, UFAL; LIZIANE MARIA DE LIMA, CNPA; REGINALDO CARVALHO, UFRPE; PÉRICLES A. MELO FILHO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
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Título: |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
African Journal of Biotechnology, v. 15, n. 30, p. 1597-1607, July 2016. |
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ISSN: |
1684-5315 |
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Idioma: |
Inglês |
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Conteúdo: |
Essential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut. MenosEssential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a sh... Mostrar Tudo |
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Palavras-Chave: |
Aranha ácaro; Ocimum Basicilum L; Spider mites. |
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Thesagro: |
Amendoim; Cromatografia; Tetranychus urticae. |
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Thesaurus Nal: |
Chromatography; Eugenol; Peanuts. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156940/1/Bioactivity-of-basil.pdf
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Marc: |
LEADER 02601naa a2200325 a 4500
001 2065905
005 2017-03-03
008 2016 bl uuuu u00u1 u #d
022 $a1684-5315
100 1 $aMARTINS, M. I. G.
245 $aBioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut.$h[electronic resource]
260 $c2016
520 $aEssential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut.
650 $aChromatography
650 $aEugenol
650 $aPeanuts
650 $aAmendoim
650 $aCromatografia
650 $aTetranychus urticae
653 $aAranha ácaro
653 $aOcimum Basicilum L
653 $aSpider mites
700 1 $aSANT'ANA, A. E. G.
700 1 $aVASCONCELOS, F. M. T.
700 1 $aSILVA, W. L.
700 1 $aLIMA, L. M. de
700 1 $aCARVALHO, R.
700 1 $aMELO FILHO, P. A.
700 1 $aSANTOS, R. C. dos
773 $tAfrican Journal of Biotechnology$gv. 15, n. 30, p. 1597-1607, July 2016.
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Registro original: |
Embrapa Algodão (CNPA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
26/03/2012 |
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Data da última atualização: |
26/03/2012 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
DOMIT, L. A.; CARVALHO, D. de O.; ROCHA, L. M. P. da; BALBINO, L. C.; VASCONCELLOS, J. H.; SILVA, A. F. da; GARCIA, J. C.; KICHEL, A. N. |
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Afiliação: |
LINEU ALBERTO DOMIT, CPAMT; DIEGO DE OLIVEIRA CARVALHO, CNPMS; LEONARDO MELO PEREIRA DA ROCHA, CNPMS; LUIZ CARLOS BALBINO, CPAC; JOSE HEITOR VASCONCELLOS, CNPMS; ALEXANDRE FERREIRA DA SILVA, CNPMS; JOAO CARLOS GARCIA, CNPMS; ARMINDO NEIVO KICHEL, CNPGC. |
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Título: |
Transferências de conhecimentos e tecnologia em ILPF. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: BALBINO, L. C.; BARCELLOS, A. de O.; STONE, L. F. (Ed.). Marco referencial: integração lavoura-pecuária-floresta. Brasília, DF: Embrapa, 2011. |
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Páginas: |
p. 103-109. |
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Idioma: |
Português |
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Notas: |
Texto em português e inglês. |
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Palavras-Chave: |
ILPF; Integração lavoura pecuária floresta. |
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Thesagro: |
Transferência de Tecnologia. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 00836naa a2200253 a 4500
001 1920357
005 2012-03-26
008 2011 bl uuuu u00u1 u #d
100 1 $aDOMIT, L. A.
245 $aTransferências de conhecimentos e tecnologia em ILPF.
260 $c2011
300 $ap. 103-109.
500 $aTexto em português e inglês.
650 $aTransferência de Tecnologia
653 $aILPF
653 $aIntegração lavoura pecuária floresta
700 1 $aCARVALHO, D. de O.
700 1 $aROCHA, L. M. P. da
700 1 $aBALBINO, L. C.
700 1 $aVASCONCELLOS, J. H.
700 1 $aSILVA, A. F. da
700 1 $aGARCIA, J. C.
700 1 $aKICHEL, A. N.
773 $tIn: BALBINO, L. C.; BARCELLOS, A. de O.; STONE, L. F. (Ed.). Marco referencial: integração lavoura-pecuária-floresta. Brasília, DF: Embrapa, 2011.
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