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| Registros recuperados : 81 | |
| 23. |  | DRUMOND, M. A.; POGGIANI, F. Distribuicao da biomassa e dos nutrientes em plantacoes puras e consorciadas de Liquidambar stylaciflua e Pinus caribaea hondurensis. In: CONGRESSO FLORESTAL PANAMERICANO, 1.; CONGRESSO FLORESTAL BRASILEIRO, 7., 1993, Curitiba. Floresta para o Desenvolvimento: Política, Ambiente, Tecnologia e Mercado: anais. São Paulo: SBS; [S.l.]: SBEF, 1993. v.1, p.234-239.| Biblioteca(s): Embrapa Florestas. |
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| 24. | | SCHUMACHER, M. V.; POGGIANI, F. Caracterização microclimática no interior dos talhoes de Eucalyptus camaldulensis Dehnh, Eucalyptus grandis Hill ex Maiden e Eucalyptus torelliana F. Muell, localizados em Anhembi-SP. Ciência Florestal, Santa Maria, v. 3, n. 1, p .9-20, nov. ,1993.| Biblioteca(s): Embrapa Florestas. |
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| 26. | | POGGIANI, F.; MONTEIRO, C. C. Efeito da implantação de maciços florestais puros na reabilitação do solo degradado pela mineração de xisto betuminoso. Silvicultura, São Paulo, v. 12, n. 42, t. 3, p. 275-281, 1992. Edição dos Anais do Congresso Florestal Brasileiro, 6., 1990, Campos do Jordão.| Biblioteca(s): Embrapa Florestas. |
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| 29. | | DRUMOND, M. A.; POGGIANI, F. Determinacao do indice de area foliar de plantacoes puras e mistas de Liquidambar styracifina e Pinus caribae var hondurensis. In: CONGRESSO FLORESTAL PANAMERICANO, 1.; CONGRESSO FLORESTAL BRASILEIRO, 7., 1993, Curitiba. Floresta para o Desenvolvimento: Política, Ambiente, Tecnologia e Mercado: anais. São Paulo: SBS; [S.l.]: SBEF, 1993. v.2, p.737.| Biblioteca(s): Embrapa Florestas. |
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| 31. | | POGGIANI, F.; SIMOES, J. W. Influencia das especies usadas no reflorestamento e da proximidade de um fragmento florestal na regeneracao do sub-bosque em areas degradadas pela mineracao. In: CONGRESSO FLORESTAL PANAMERICANO, 1.; CONGRESSO FLORESTAL BRASILEIRO, 7., 1993, Curitiba. Floresta para o Desenvolvimento: Política, Ambiente, Tecnologia e Mercado: anais. São Paulo: SBS; [S.l.]: SBEF, 1993. v. 1, p. 50-54.| Biblioteca(s): Embrapa Florestas. |
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| 35. | | POGGIANI, F.; MONTEIRO JUNIOR, E. dos. Deposição de folhedo e retorno de nutrientes ao solo numa floresta estacional semidecídua em Piracicaba (Estado de São Paulo). Silvicultura, São Paulo, v. 12, n. 42, t. 3, p. 596-602, 1992. Edição dos Anais do Congresso Florestal Brasileiro, 6., 1990, Campos do Jordão.| Biblioteca(s): Embrapa Florestas. |
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| 40. | | NOVAES, R. F. F.; POGGIANI, F. Efeito da consorciação entre Pinus caribaea var. hondurensis e Liquidambar styraciflua L., sobre a ciclagem de nutrientes em florestas implantadas. Silvicultura, São Paulo, v. 8, n. 28, p. 400-403, 1983. Edição dos anais do 4º Congresso Florestal Brasileiro, 1982, Belo Horizonte.| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 81 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
03/02/2014 |
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Data da última atualização: |
10/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. |
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Afiliação: |
Maria Beatriz Calderan Rodrigues, USP-ESALQ; LEIA CECILIA DE LIMA FAVARO, CNPAE; Ana Paula de Souza Pallu, USP-ESALQ; ANDERSON FERREIRA, CPAMT; Fernanda de Souza Sebastianes, USP-ESALQ; Maria Juliana Calderan Rodrigues, USP-ESALQ; Marcel Bellato Spósito, USP-ESALQ; Welington Luiz DE Araújo, UMC; Aline Aparecida Pizzirani-Kleiner, USP-ESALQ. |
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Título: |
Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013. |
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ISSN: |
1878-6146 |
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DOI: |
http://dx.doi.org/10.1016/j.funbio.2013.06.005 |
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Idioma: |
Inglês |
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Conteúdo: |
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction. MenosGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for ... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium-Mediated; Plant-Pathogen Interaction. |
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Thesaurus NAL: |
citrus black spot; genetic transformation; Guignardia citricarpa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96268/1/cpamt-Ferreira-1878-6146-2013.pdf
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Marc: |
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520 $aGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction.
650 $acitrus black spot
650 $agenetic transformation
650 $aGuignardia citricarpa
653 $aAgrobacterium-Mediated
653 $aPlant-Pathogen Interaction
700 1 $aFAVARO, L. C. de L.
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700 1 $aPIZZIRANI-KLEINER, A. A.
773 $tFungal Biology, Oxford$gv. 117, n. 7/8, p. 556-568, jul./aug., 2013.
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