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| 1. |  | REGINA, Q.; OLIVEIRA, Y. de; PILEGGI, M.; CUQUEL, F.; QUOIRIN, M. Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis cotiledonary culture. Brazilian Archives of Biology and Technology, Curitiba, v. 52, n. 6, p. 1485-1492, Nov./Dec. 2009.| Biblioteca(s): Embrapa Florestas. |
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| 2. | | KALIL, G. P. da C.; CHRESTANI, F.; RAUPP, D. da S.; CLEMENTE, E.; KALIL FILHO, A. N.; PILEGGI, M.; HANSEL, F. A. Dinâmica do ph e taxa de crescimento de fungos mesófilos e enterobactérias psicrófilas durante o armazenamento de palmito de pupunha minimamente processado. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 6., 2005, Campinas. Ciência de alimentos: abrindo caminhos para o desenvolvimento científico, tecnológico e industrial. [S.l.]: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2005. Resumo.| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/09/2023 |
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Data da última atualização: |
14/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
GONÇALVES, M. S.; FARIA, J. P. J.; SILVA, J. R.; CUSTÓDIO, D. A. C.; RIBEIRO, J. B.; GUIMARÃES, A. S.; DORNELES, E. M. S.; COSTA, G. M. |
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Afiliação: |
MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; JAMILA P. J. FARIA, UNIVERSIDADE FEDERAL DE LAVRAS; JULIANA R. SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; DIRCÉIA A. C. CUSTÓDIO, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE M. S. DORNELES, UNIVERSIDADE FEDERAL DE LAVRAS; GERALDO M. COSTA, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Flagella are an important virulence factor in the subclinical persistence of Escherichia coli in bovine mammary gland. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Dairy Research, v. 90, n. 2, p. 146-151, 2023. |
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DOI: |
https://doi.org/10.1017/S0022029923000353 |
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Idioma: |
Inglês |
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Conteúdo: |
We compared the virulence profile and REP-PCR genotypes of Escherichia coli strains isolated from subclinical and clinical mastitis cases and dairy farm environments in Minas Gerais State, Brazil, to determine virulence factors and genotypes potentially associated with subclinical persistence in the udder. The virulence profile was obtained by the search for three virulence genes: lpfA (long polar fimbriae), fliC (flagella), and escN (type III secretion system). Subclinical isolates exhibited mainly the fliC gene (33.33%) and fliC + escN genes (30.30%). Clinical isolates exhibited mainly fliC + escN genes (50%) and environmental isolates the lpfA + escN genes (58.04%). Strains isolated from subclinical mastitis showed 6.75 times more positivity to fliC than environmental isolates. Thirty-four genotypes were observed in the REP-PCR analysis, and clinical mastitis isolates indicated more genetic proximity to dairy farm environment isolates than subclinical mastitis isolates. In conclusion, the results suggested that flagella may be an important virulence factor for mammary persistent E. coli infection in cattle, however, none of the E. coli REP-PCR genotypes were associated with subclinical infection. |
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Palavras-Chave: |
Mastite; MPEC; REP-PCR. |
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Thesagro: |
Bovino; Doença Animal; Gado Leiteiro. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02086naa a2200289 a 4500
001 2156330
005 2023-12-14
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0022029923000353$2DOI
100 1 $aGONÇALVES, M. S.
245 $aFlagella are an important virulence factor in the subclinical persistence of Escherichia coli in bovine mammary gland.$h[electronic resource]
260 $c2023
520 $aWe compared the virulence profile and REP-PCR genotypes of Escherichia coli strains isolated from subclinical and clinical mastitis cases and dairy farm environments in Minas Gerais State, Brazil, to determine virulence factors and genotypes potentially associated with subclinical persistence in the udder. The virulence profile was obtained by the search for three virulence genes: lpfA (long polar fimbriae), fliC (flagella), and escN (type III secretion system). Subclinical isolates exhibited mainly the fliC gene (33.33%) and fliC + escN genes (30.30%). Clinical isolates exhibited mainly fliC + escN genes (50%) and environmental isolates the lpfA + escN genes (58.04%). Strains isolated from subclinical mastitis showed 6.75 times more positivity to fliC than environmental isolates. Thirty-four genotypes were observed in the REP-PCR analysis, and clinical mastitis isolates indicated more genetic proximity to dairy farm environment isolates than subclinical mastitis isolates. In conclusion, the results suggested that flagella may be an important virulence factor for mammary persistent E. coli infection in cattle, however, none of the E. coli REP-PCR genotypes were associated with subclinical infection.
650 $aBovino
650 $aDoença Animal
650 $aGado Leiteiro
653 $aMastite
653 $aMPEC
653 $aREP-PCR
700 1 $aFARIA, J. P. J.
700 1 $aSILVA, J. R.
700 1 $aCUSTÓDIO, D. A. C.
700 1 $aRIBEIRO, J. B.
700 1 $aGUIMARÃES, A. S.
700 1 $aDORNELES, E. M. S.
700 1 $aCOSTA, G. M.
773 $tJournal of Dairy Research$gv. 90, n. 2, p. 146-151, 2023.
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