Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
27/07/2009 |
Data da última atualização: |
26/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MENNA, P.; PEREIRA, A. A.; BANGEL, E. V.; HUNGRIA, M. |
Afiliação: |
PÂMELA MENNA, UEL/CNPq-MCT; ALAN ALVES PEREIRA, CNPq-MCT; ELIANE VILLAMIL BANGEL, FEPAGRO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
Título: |
Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Symbiosis, Philadelphia, v. 48, n. 1-3, p. 120-130, Feb. 2009. |
Idioma: |
Inglês |
Conteúdo: |
With more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach ? 20%∶80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups ? might expedite rhizobial diversity studies. |
Thesagro: |
Bactéria; Fixação de Nitrogênio; Inoculante; Taxonomia. |
Thesaurus Nal: |
Inoculation methods; Nitrogen fixation. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 01873naa a2200229 a 4500 001 1471352 005 2024-06-26 008 2009 bl uuuu u00u1 u #d 100 1 $aMENNA, P. 245 $aRep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool.$h[electronic resource] 260 $c2009 520 $aWith more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach ? 20%∶80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups ? might expedite rhizobial diversity studies. 650 $aInoculation methods 650 $aNitrogen fixation 650 $aBactéria 650 $aFixação de Nitrogênio 650 $aInoculante 650 $aTaxonomia 700 1 $aPEREIRA, A. A. 700 1 $aBANGEL, E. V. 700 1 $aHUNGRIA, M. 773 $tSymbiosis, Philadelphia$gv. 48, n. 1-3, p. 120-130, Feb. 2009.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|