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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
03/09/2007 |
Data da última atualização: |
30/03/2015 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
SILVEIRA, E. R.; PELISSARI, A.; BONATTO, S. R.; MORAES, A. de; OLIVEIRA, E. B. de. |
Afiliação: |
Edson Roberto Silveira, UTFPR; Adelino Pelissari, UFPR; Sionei Ricardo Bonatto, Bolsista CNPq; Aníbal de Moraes, UFPR; Edílson Batista de Oliveira, Embrapa Florestas. |
Título: |
Artrópodos predadores e fitófagos em milho em sistema de integração lavoura-pecuária. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL INTEGRAÇÃO LAVOURA-PECUÁRIA, 2007, Curitiba. Simpósio... [Curitiba]: UFPR; [S.l.]: UFRGS; [S.l.]: Ohio State University, 2007. |
Páginas: |
3 p. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Herbívoro. |
Thesagro: |
Inimigo Natural; Inseto; Zea Mays. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/120211/1/digitalizar0022.pdf
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Marc: |
LEADER 00723nam a2200205 a 4500 001 1313360 005 2015-03-30 008 2007 bl uuuu u00u1 u #d 100 1 $aSILVEIRA, E. R. 245 $aArtrópodos predadores e fitófagos em milho em sistema de integração lavoura-pecuária.$h[electronic resource] 260 $aIn: SIMPÓSIO INTERNACIONAL INTEGRAÇÃO LAVOURA-PECUÁRIA, 2007, Curitiba. Simpósio... [Curitiba]: UFPR; [S.l.]: UFRGS; [S.l.]: Ohio State University$c2007 300 $a3 p.$c1 CD-ROM. 650 $aInimigo Natural 650 $aInseto 650 $aZea Mays 653 $aHerbívoro 700 1 $aPELISSARI, A. 700 1 $aBONATTO, S. R. 700 1 $aMORAES, A. de 700 1 $aOLIVEIRA, E. B. de
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Amazônia Ocidental. |
Data corrente: |
06/03/2023 |
Data da última atualização: |
01/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 3 |
Autoria: |
CASTRO, G. S.; SOUZA, T. F.; SILVA, G. F. da; PEDROSO, R. C. N.; MENEZES, K. S.; SOARES, M. A.; DIAS, G. M.; SANTOS, A. O.; YAMAGISHI, M. E. B.; FARIA, J. V.; JANUÁRIO, A. H.; KOOLEN, H. H. F. |
Afiliação: |
GLEUCINEI S. CASTRO, UNIVERSIDADE DO ESTADO DO AMAZONAS; THIAGO F. SOUSA, UNIVERSIDADE FEDERAL DO AMAZONAS; GILVAN FERREIRA DA SILVA, CPAA; RITA C. N. PEDROSO, UNIVERSIDADE DE FRANCA; KELLY S. MENEZES, UNIVERSIDADE DO ESTADO DO AMAZONAS; MARCOS A. SOARES, UNIVERSIDADE FEDERAL DE MATO GROSSO; GUSTAVO M. DIAS, UNIVERSIDADE FEDERAL DO ABC; ALINE O. SANTOS, UNIVERSIDADE DO ESTADO DO AMAZONAS; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; JÉSSICA V. FARIA, UNIVERSIDADE DO ESTADO DO AMAZONAS; ANA H. JANUÁRIO, UNIVERSIDADE DE FRANCA; HECTOR H. F. KOOLEN, UNIVERSIDADE DO ESTADO DO AMAZONAS. |
Título: |
Characterization of peptaibols produced by a marine strain of the fungus Trichoderma endophyticum via Mass Spectrometry, genome mining and phylogeny-based prediction. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Metabolites, v. 13, n. 2, 221, Feb. 2023. |
DOI: |
https://doi.org/10.3390/metabo13020221 |
Idioma: |
Inglês |
Conteúdo: |
Abstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for peptaibol diversification. This study demonstrates the importance of combining genome mining techniques, mass spectrometry analysis and molecular networks for the discovery of new natural products. MenosAbstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for pept... Mostrar Tudo |
Palavras-Chave: |
De novo sequencing; Fungo marinho; Marine fungus; Molecular networking; Nonribosomal peptides; Peptídeos não ribossomais; Rede molecular; Synteny analysis; Tricoderma. |
Thesagro: |
Peptídeo. |
Thesaurus NAL: |
Hypocreaceae. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152102/1/metabolites-13-00221-v2.pdf
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Marc: |
LEADER 02911naa a2200397 a 4500 001 2152102 005 2023-09-01 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/metabo13020221$2DOI 100 1 $aCASTRO, G. S. 245 $aCharacterization of peptaibols produced by a marine strain of the fungus Trichoderma endophyticum via Mass Spectrometry, genome mining and phylogeny-based prediction.$h[electronic resource] 260 $c2023 520 $aAbstract. Trichoderma is recognized as a prolific producer of nonribosomal peptides (NRPs) known as peptaibols, which have remarkable biological properties, such as antimicrobial and anticancer activities, as well as the ability to promote systemic resistance in plants against pathogens. In this study, the sequencing of 11-, 14- and 15-res peptaibols produced by a marine strain of Trichoderma isolated from the ascidian Botrylloides giganteus was performed via liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). Identification, based on multilocus phylogeny, revealed that our isolate belongs to the species T. endophyticum, which has never been reported in marine environments. Through genome sequencing and genome mining, 53 biosynthetic gene clusters (BGCs) were identified as being related to bioactive natural products, including two NRP-synthetases: one responsible for the biosynthesis of 11- and 14-res peptaibols, and another for the biosynthesis of 15-res. Substrate prediction, based on phylogeny of the adenylation domains in combination with molecular networking, permitted extensive annotation of the mass spectra related to two new series of 15-res peptaibols, which are referred to herein as "endophytins". The analyses of synteny revealed that the origin of the 15-module peptaibol synthetase is related to 18, 19 and 20-module peptaibol synthetases, and suggests that the loss of modules may be a mechanism used by Trichoderma species for peptaibol diversification. This study demonstrates the importance of combining genome mining techniques, mass spectrometry analysis and molecular networks for the discovery of new natural products. 650 $aHypocreaceae 650 $aPeptídeo 653 $aDe novo sequencing 653 $aFungo marinho 653 $aMarine fungus 653 $aMolecular networking 653 $aNonribosomal peptides 653 $aPeptídeos não ribossomais 653 $aRede molecular 653 $aSynteny analysis 653 $aTricoderma 700 1 $aSOUZA, T. F. 700 1 $aSILVA, G. F. da 700 1 $aPEDROSO, R. C. N. 700 1 $aMENEZES, K. S. 700 1 $aSOARES, M. A. 700 1 $aDIAS, G. M. 700 1 $aSANTOS, A. O. 700 1 $aYAMAGISHI, M. E. B. 700 1 $aFARIA, J. V. 700 1 $aJANUÁRIO, A. H. 700 1 $aKOOLEN, H. H. F. 773 $tMetabolites$gv. 13, n. 2, 221, Feb. 2023.
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Embrapa Amazônia Ocidental (CPAA) |
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