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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
07/04/2005 |
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Data da última atualização: |
07/04/2005 |
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Autoria: |
OLIVEIRA, M. G. de; GOMES, D. M. S.; MOURA, M. V. L. P. |
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Título: |
Anatomia e micromorfologia foliar de Melanopsidium nigrum Colla. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Floresta e Ambiente, Rio de Janeiro, v. 10, n. 2, p. 87-94, ago./dez. 2003. |
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Idioma: |
Português |
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Palavras-Chave: |
Anatomia foliar; Melanopsidium nigrum. |
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Thesagro: |
Rubiaceae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00513naa a2200169 a 4500
001 1310508
005 2005-04-07
008 2003 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, M. G. de
245 $aAnatomia e micromorfologia foliar de Melanopsidium nigrum Colla.
260 $c2003
650 $aRubiaceae
653 $aAnatomia foliar
653 $aMelanopsidium nigrum
700 1 $aGOMES, D. M. S.
700 1 $aMOURA, M. V. L. P.
773 $tFloresta e Ambiente, Rio de Janeiro$gv. 10, n. 2, p. 87-94, ago./dez. 2003.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
07/02/2019 |
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Data da última atualização: |
07/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GUIMARÃES, M. Z. P.; DE VECCHI, R.; VITÓRIA, G.; SOCHACKI, J. K.; PAULSEN, B. S.; LIMA, I.; SILVA, F. R. da; COSTA, R. F. M. da; CASTRO, N. G.; BRETON, L.; REHEN, S. K. |
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Afiliação: |
MARÍLIA Z. P. GUIMARÃES, D’Or Institute for Research and Education, UFRJ; RODRIGO DE VECCHI, L’Oréal Research & Innovation, Unicamp; GABRIELA VITÓRIA, L’Oréal Research & Innovation; JAROSLAW K. SOCHACKI, L’Oréal Research & Innovation; BRUNA S. PAULSEN, L’Oréal Research & Innovation; IGOR LIMA, L’Oréal Research & Innovation; FELIPE RODRIGUES DA SILVA, CNPTIA, Unicamp; RODRIGO F. M. DA COSTA, L’Oréal Research & Innovation; NEWTON G. CASTRO, UFRJ; LIONEL BRETON, L’Oréal Research & Innovation; STEVENS K. REHEN, D’Or Institute for Research and Education, UFRJ. |
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Título: |
Generation of iPSC-derived human peripheral sensory neurons releasing substance P elicited by TRPV1 agonists. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Frontiers in Molecular Neuroscience, v. 11, p. 1-17, Aug. 2018. |
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DOI: |
10.3389/fnmol.2018.00277 |
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Idioma: |
Inglês |
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Notas: |
Article 277. |
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Conteúdo: |
Neural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs and peripheral sensory neurons (PSNs) from human induced pluripotent stem cells (hiPSCs), generated sensory cells without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation into NCPCs and functional PSNs using chemically defined media and factors to achieve efficient differentiation, confirmed by the expression of specific markers. After 10 days hiPSCs differentiated into NCPCs, cells were then maintained in neural induction medium containing defined growth factors for PSNs differentiation, followed by 10 days in neonatal human epidermal keratinocytes- (HEKn-) conditioned medium (CM). We observed a further increase in PSN markers expression and neurites length after CM treatment. The resulting neurons elicited action potentials after current injection and released substance P (SP) in response to nociceptive agents such as anandamide and resiniferatoxin. Anandamide induced substance P release via activation of TRPV1 and not CB1. Transcriptomic analysis of the PSNs revealed the main dorsal root ganglia neuronal markers and a transcriptional profile compatible with C fiber-low threshold mechanoreceptors. TRPV1 was detected by immunofluorescence and RNA-Seq in multiple experiments. In conclusion, the developed strategy generated PSNs useful for drug screening that could be applied to patient-derived hiPSCs, consisting in a powerful tool to model human diseases in vitro. MenosNeural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs and peripheral sensory neurons (PSNs) from human induced pluripotent stem cells (hiPSCs), generated sensory cells without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation into NCPCs and functional PSNs using chemically defined media and factors to achieve efficient differentiation, confirmed by the expression of specific markers. After 10 days hiPSCs differentiated into NCPCs, cells were then maintained in neural induction medium containing defined growth factors for PSNs differentiation, followed by 10 days in neonatal human epidermal keratinocytes- (HEKn-) conditioned medium (CM). We observed a further increase in PSN markers expression and neurites length after CM treatment. The resulting neurons elicited action potentials after current injection and released substance P (SP) in response to nociceptive agents such as anandamide and resiniferatoxin. Anandamide induced substance P release via activation of TRPV1 and not CB1. Transcriptomic analysis of the PSNs revealed the main dorsal root ganglia neuronal markers and a transcriptional profile compatible with C fiber-low threshold mechanoreceptors. TRPV1 was detected by immunofluorescence and RNA-Seq in multiple experiments. In conclusion, the developed strategy generated P... Mostrar Tudo |
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Palavras-Chave: |
Human induced pluripotent stem cells; Neural crest stem cells; Neurônios sensoriais; Pré-clínico; Substância P; TRPV1. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192302/1/AP-Generation-iPSC-Felipe.pdf
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Marc: |
LEADER 02655naa a2200337 a 4500
001 2105696
005 2019-02-07
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fnmol.2018.00277$2DOI
100 1 $aGUIMARÃES, M. Z. P.
245 $aGeneration of iPSC-derived human peripheral sensory neurons releasing substance P elicited by TRPV1 agonists.$h[electronic resource]
260 $c2018
500 $aArticle 277.
520 $aNeural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs and peripheral sensory neurons (PSNs) from human induced pluripotent stem cells (hiPSCs), generated sensory cells without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation into NCPCs and functional PSNs using chemically defined media and factors to achieve efficient differentiation, confirmed by the expression of specific markers. After 10 days hiPSCs differentiated into NCPCs, cells were then maintained in neural induction medium containing defined growth factors for PSNs differentiation, followed by 10 days in neonatal human epidermal keratinocytes- (HEKn-) conditioned medium (CM). We observed a further increase in PSN markers expression and neurites length after CM treatment. The resulting neurons elicited action potentials after current injection and released substance P (SP) in response to nociceptive agents such as anandamide and resiniferatoxin. Anandamide induced substance P release via activation of TRPV1 and not CB1. Transcriptomic analysis of the PSNs revealed the main dorsal root ganglia neuronal markers and a transcriptional profile compatible with C fiber-low threshold mechanoreceptors. TRPV1 was detected by immunofluorescence and RNA-Seq in multiple experiments. In conclusion, the developed strategy generated PSNs useful for drug screening that could be applied to patient-derived hiPSCs, consisting in a powerful tool to model human diseases in vitro.
653 $aHuman induced pluripotent stem cells
653 $aNeural crest stem cells
653 $aNeurônios sensoriais
653 $aPré-clínico
653 $aSubstância P
653 $aTRPV1
700 1 $aDE VECCHI, R.
700 1 $aVITÓRIA, G.
700 1 $aSOCHACKI, J. K.
700 1 $aPAULSEN, B. S.
700 1 $aLIMA, I.
700 1 $aSILVA, F. R. da
700 1 $aCOSTA, R. F. M. da
700 1 $aCASTRO, N. G.
700 1 $aBRETON, L.
700 1 $aREHEN, S. K.
773 $tFrontiers in Molecular Neuroscience$gv. 11, p. 1-17, Aug. 2018.
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