Data/hora: 23/09/2024 19:47:58

Biblioteca(s): 	Embrapa Instrumentação.	
Data corrente: 	14/10/2020	
Data da última atualização: 	12/08/2022	
Autoria: 	CÔNSOLO, N. R. B.; SAMUELSSON, L. M.; BARBOSA, L. C. G. S.; MONARETTO, T.; MORAES, T. B.; BUARQUE, V. L. M.; HIGUERA-PADILLA, A. R.; COLNAGO, L. A.; SILVA, S. L.; REIS, M. M.; FONSECA, A. C.; ARAÚJO, C. S. S.; LEITE, B. G. S.; ROQUE, F. A.; ARAÚJO, L. F.	
Afiliação: 	LUIZ ALBERTO COLNAGO, CNPDIA.	
Título: 	Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Poultry Science, v. 99, n. 11, 2020.	
Páginas: 	6247-6257	
DOI: 	https://doi.org/10.1016/j.psj.2020.06.066	
Idioma: 	Inglês	
Conteúdo: 	Metabolite profiles of chicken breast extracts and water mobility in breasts were studied using proton nuclear magnetic resonance (1H-NMR) spectroscopy and time-domain NMR (TD-NMR) relaxometry, respectively, using normal breast (NB), and wooden breast (WB) and white striping (WS) myopathies in broilers. One thousand eight hundred sixty broilers were raised to commercial standards, receiving the same diets that were formulated as per the different growth stages. At 49 D of age, 200 animals were slaughtered following routine commercial procedures, and at 4 h postmortem, the whole breast (pectoralis major muscle) was removed and visually inspected by an experienced meat inspector who selected NB (without myopathies) and samples with the presence of WS and WB myopathies. Fifteen breasts (5 each of NB, WS, and WB) were analyzed through TD-NMR relaxometry, and samples of approximately 20 g were taken from each breast and frozen at −80°C for metabolite profiling through 1H-NMR spectroscopy. Multivariate statistical analysis was used to evaluate the effect on water relaxometry and metabolite profile in accordance with the presence and type of myopathy in the breast. 1H-NMR data showed that the metabolite profiles in WS and WB breasts were different from each other and from NB. This pilot study shows that myopathies appear to be related to hypoxia, connective tissue deposition, lower mitochondrial function, and greater oxidative stress compared with NB. The longitudinal and transverse relaxation time of the breasts determined by TD-NMR relaxometry was shorter for NB than that for WS and WB, indicating greater water mobility in breasts affected by myopathies. 1H-NMR spectroscopy can be used to differentiate the metabolism of WS, WB, and NB, and TD-NMR has the potential to be a fast, simple, and noninvasive method to distinguish NB from WB and WS. As a practical application, the metabolomic profile as per the occurrence of breast myopathies may be used for a better understanding of these issues, which opens a gap to mitigate the incidence and severity of WS and WB. In addition, the present study brings an opportunity for the development of a new and objective tool to classify the incidence of breast myopathies through TD-NMR relaxometry.	
Palavras-Chave: 	Chicken breast myopathy; NMR spectroscopy; TD-NMR relaxometry; Water mobilization.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Instrumentação (CNPDIA)	
Exemplares: 	CNPDIA - (17618-1) - UPC - AP - DD - PROCI.20/095 -  - 2020/0103 - DIS