|
Registro Completo |
|
Biblioteca(s): |
Embrapa Florestas. |
|
Data corrente: |
24/09/2003 |
|
Data da última atualização: |
11/07/2011 |
|
Autoria: |
LIMA, G. P. P.; PIZA, I. M. T.; HENRIQUE, A.; TAKAKI, M. |
|
Título: |
Polyamines as salinity biochemical marker in callus of Eucalyptus urograndis. |
|
Ano de publicação: |
2003 |
|
Fonte/Imprenta: |
Ciência Florestal, Santa Maria, v. 13, n. 1, p. 43-48, jun. 2003. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Biochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity. |
|
Palavras-Chave: |
Estresse salino; Poliaminas. |
|
Thesagro: |
Micropropagação. |
|
Thesaurus Nal: |
Eucalyptus; micropropagation; polyamines; salt stress. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01534naa a2200241 a 4500
001 1307189
005 2011-07-11
008 2003 bl uuuu u00u1 u #d
100 1 $aLIMA, G. P. P.
245 $aPolyamines as salinity biochemical marker in callus of Eucalyptus urograndis.
260 $c2003
520 $aBiochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.
650 $aEucalyptus
650 $amicropropagation
650 $apolyamines
650 $asalt stress
650 $aMicropropagação
653 $aEstresse salino
653 $aPoliaminas
700 1 $aPIZA, I. M. T.
700 1 $aHENRIQUE, A.
700 1 $aTAKAKI, M.
773 $tCiência Florestal, Santa Maria$gv. 13, n. 1, p. 43-48, jun. 2003.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Florestas (CNPF) |
|
