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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
04/10/2016 |
Data da última atualização: |
29/12/2017 |
Autoria: |
SOUSA, P. C.; SANTOS, E. A. A.; SILVA, A. M.; BEZERRA, J. A. B.; SOUZA, A. L. P.; LIMA, G. L.; OLIVEIRA, M. F.; SILVA, A. R. |
Afiliação: |
PATRÍCIA C. SOUSA, UFERSA; ERIKA A. A. SANTOS, UFERSA; ANDRÉIA M. SILVA, UFERSA; JOSÉ A. B. BEZERRA, UFERSA; ANA L. P. SOUZA, UFERSA; GABRIELA L. LIMA, UFRR; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA. |
Título: |
Identification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 8, p. 767-774, ago. 2016. |
Idioma: |
Inglês |
Conteúdo: |
The aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol. MenosThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented da... Mostrar Tudo |
Palavras-Chave: |
Dano ultraestrutural e funcional; Euphractus sexcinctus; Membrane integrity; Mitochondrial activity; Six-banded armadillo; Sperm; Tatu-peba; Ultraestrutura; Ultrastructural and functional damages; Xenarthras. |
Thesagro: |
Criopreservação; Espermatozoide; Membrana; Mitocondria; Sêmen. |
Thesaurus Nal: |
Cryopreservation; Spermatozoa; Ultrastructure. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/148299/1/Identification-of-ultrastructural.pdf
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Marc: |
LEADER 03089naa a2200421 a 4500 001 2054060 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aSOUSA, P. C. 245 $aIdentification of ultrastructural and functional damage in sperm from six-banded armadillos (Euphractus sexcinctus) due to cryopreservation. 260 $c2016 520 $aThe aim of the study was to cryopreserve the semen of six-banded armadillos (Euphractus sexcinctus) in Tris-yolk and glycerol diluent, and to determine the damage caused by the freezing-thawing process, using fluorescent markers and ultrastructural analysis. Semen samples (n=11) collected from 4 adult six-banded armadillos by electroejaculation were cryopreserved in Tris diluent plus 20% egg yolk and 3% glycerol, in a fast freezing curve. Classical analysis of samples was performed after dilution, refrigeration and thawing, followed by fluorescence analysis, using a combination of fluorescent probes to assess membrane integrity (propidium iodide - PI and Hoechst - H342), and mitochondrial activity (CMXRos - Mito Tracker Red®). We also used the ultrastructural analysis to verify possible morphological alterations caused by cryoinjuries. When compared with fresh samples, we verified a significant decline in all the armadillos? semen parameters after thawing, in which only 6.1% motile sperm were found. However, the percentage of sperm which remained with viable (13%) and functional (24.7%) membranes after thawing suggests that some cells could be live but immotile. Analysis using fluorescent markers revealed that the mitochondria of armadillos? sperm is highly sensible to the freezing protocol and the findings through ultrastructure analysis proved this statement. Additionally, the images obtained by transmission electron microscopy revealed that frozenthawed sperm presented damaged plasma membrane, nuclear modifications as changes in chromatin and acrossomal changes relative to sperm capacitation. In conclusion, this study is the first attempt to cryopreserve the semen of an armadillo species, and to help us to identify critical points on the freezing-thawing procedure in order to improve the protocol. 650 $aCryopreservation 650 $aSpermatozoa 650 $aUltrastructure 650 $aCriopreservação 650 $aEspermatozoide 650 $aMembrana 650 $aMitocondria 650 $aSêmen 653 $aDano ultraestrutural e funcional 653 $aEuphractus sexcinctus 653 $aMembrane integrity 653 $aMitochondrial activity 653 $aSix-banded armadillo 653 $aSperm 653 $aTatu-peba 653 $aUltraestrutura 653 $aUltrastructural and functional damages 653 $aXenarthras 700 1 $aSANTOS, E. A. A. 700 1 $aSILVA, A. M. 700 1 $aBEZERRA, J. A. B. 700 1 $aSOUZA, A. L. P. 700 1 $aLIMA, G. L. 700 1 $aOLIVEIRA, M. F. 700 1 $aSILVA, A. R. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 8, p. 767-774, ago. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
28/10/2010 |
Data da última atualização: |
16/07/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
TEIXEIRA, J. de M, A.; MOURA, J. M.; SILVA. U. C.; CALAZANS, G. M.; OLIVEIRA, A. C. de; MARRIEL, I. E. |
Afiliação: |
JAQUELINE DE MOURA ARAUJO TEIXEIRA, UNIFEMM; JOSIANE M. MOURA, UNIFEMM; UBIANA CASSIA SILVA, UNIFEMM; GIOVANNA MOURA CALAZANS, UNIFEMM; ANTONIO CARLOS DE OLIVEIRA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS. |
Título: |
Estoque de nitrogênio total e relação C/N influenciados pelo sistema de manejo e uso do solo de Cerrado. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Agregação do solo; Sistemas de plantio. |
Thesagro: |
Estrutura do Solo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25163/1/0371.pdf
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Marc: |
LEADER 00819nam a2200205 a 4500 001 1865641 005 2018-07-16 008 2010 bl uuuu u00u1 u #d 100 1 $aTEIXEIRA, J. de M, A. 245 $aEstoque de nitrogênio total e relação C/N influenciados pelo sistema de manejo e uso do solo de Cerrado.$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS$c2010 300 $c1 CD-ROM. 650 $aEstrutura do Solo 653 $aAgregação do solo 653 $aSistemas de plantio 700 1 $aMOURA, J. M. 700 1 $aSILVA. U. C. 700 1 $aCALAZANS, G. M. 700 1 $aOLIVEIRA, A. C. de 700 1 $aMARRIEL, I. E.
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