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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
13/01/2009 |
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Data da última atualização: |
14/01/2009 |
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Autoria: |
CORTE, V. B.; BORGES, E. E. de L. e; VENTRELLA, M. C.; LEITE, I. T. de A. L.; BRAGA, A. J. T. |
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Título: |
Histochemical aspects of reserves mobilization of Caesalpinia peltophoroides (leguminosae) seeds during germination and seedlings early growth. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Revista Árvore, Viçosa, v. 32, n. 4, p. 631-640, jul./ago. 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to investigate reserve mobilization in Caesalpinia peltophoroides seeds during germination and initial seedling growth. The variation in these compounds was analyzed from the pre-germination period (0 to 5 days after sowing - DAS) to the total cotyledon senescence and abscission at 35 DAS. For this histochemical tests were made on cotyledons fixed in FAA50 or included in glycol-metacrylate. To follow the mobilization of the main reserve compounds, sudan III was used to detect total lipids, xylidine Ponceau to detect total proteins, lugol to detect starch and polarized light to visualize the crystals. The lipids, present in a great quantity in the cotyledon, gradually decreased in the period studied. A greater quantity of starch was observed on the 10th DAS than in the previous periods and it was totally consumed by 30 DAS. The distribution pattern and the morphology of the protein material were very modified by 10 DAS, a period during which it was intensely consumed, remaining only parietally fragments distributed, that practically disappeared at 25 DAS. The calcium oxalate druses were not consumed during the period studied, there was only crystal agglutination. |
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Palavras-Chave: |
Reserve materials. |
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Thesaurus Nal: |
calcium oxalate; carbohydrates; lipids; proteins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01929naa a2200229 a 4500
001 1315313
005 2009-01-14
008 2008 bl uuuu u00u1 u #d
100 1 $aCORTE, V. B.
245 $aHistochemical aspects of reserves mobilization of Caesalpinia peltophoroides (leguminosae) seeds during germination and seedlings early growth.
260 $c2008
520 $aThe objective of this study was to investigate reserve mobilization in Caesalpinia peltophoroides seeds during germination and initial seedling growth. The variation in these compounds was analyzed from the pre-germination period (0 to 5 days after sowing - DAS) to the total cotyledon senescence and abscission at 35 DAS. For this histochemical tests were made on cotyledons fixed in FAA50 or included in glycol-metacrylate. To follow the mobilization of the main reserve compounds, sudan III was used to detect total lipids, xylidine Ponceau to detect total proteins, lugol to detect starch and polarized light to visualize the crystals. The lipids, present in a great quantity in the cotyledon, gradually decreased in the period studied. A greater quantity of starch was observed on the 10th DAS than in the previous periods and it was totally consumed by 30 DAS. The distribution pattern and the morphology of the protein material were very modified by 10 DAS, a period during which it was intensely consumed, remaining only parietally fragments distributed, that practically disappeared at 25 DAS. The calcium oxalate druses were not consumed during the period studied, there was only crystal agglutination.
650 $acalcium oxalate
650 $acarbohydrates
650 $alipids
650 $aproteins
653 $aReserve materials
700 1 $aBORGES, E. E. de L. e
700 1 $aVENTRELLA, M. C.
700 1 $aLEITE, I. T. de A. L.
700 1 $aBRAGA, A. J. T.
773 $tRevista Árvore, Viçosa$gv. 32, n. 4, p. 631-640, jul./ago. 2008.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
16/03/2020 |
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Data da última atualização: |
24/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PARAENSE, L. C. R.; PENA, D. N.; DARNET, S. H.; RODRIGUES, S. de M.; MENEZES, I. C. de; CUNHA, E. F. M. |
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Afiliação: |
LUANY CAROLINE RIBEIRO PARAENSE, UFRA; DAYANE NASCIMENTO PENA, UFRA; SYLVAIN HENRI DARNET, UFPA; SIMONE DE MIRANDA RODRIGUES, CPATU; ILMARINA CAMPOS DE MENEZES, CPATU; ELISA FERREIRA MOURA CUNHA, CPATU. |
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Título: |
First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 47, n. 4, p. 2985-2989, fev. 2020. |
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DOI: |
https://doi.org/10.1007/s11033-020-05301-0 |
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Idioma: |
Inglês |
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Notas: |
Short Comunication. |
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Conteúdo: |
Platonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they were tested in 31 genotypes of P. insignis that belong to a germplasm bank and were sampled in the northeast of Pará State, Brazil. Thirteen primers were polymorphic and the number of alleles per loci varied from 5 (PI18 and PI27) to 2 (PI08, PI25, PI31, PI33 and PI 37). Expected heterozygosity (HE) varied from 0.74 (PI27) to 0.12 (PI31)b and observed heterozygosity (HO) varied from 1.00 (PI25) to 0.00 (PI08, PI31, PI33 and PI37). Principal coordinates could separate the genotypes of P. insignis in clusters and we can conclude that the primers can estimate the genetic diversity of P. insignis populations. |
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Palavras-Chave: |
Diversidade genética; Genetic diversity; Next generation sequencing; Sequenciamento genético; Simple sequence repeats. |
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Thesagro: |
Bacuri; Platonia Insignis. |
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Thesaurus NAL: |
Clusiaceae; Oligonucleotides. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211920/1/Paraense2020-Article-FirstGenomicMicrosatelliteMark.pdf
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Marc: |
LEADER 02375naa a2200313 a 4500
001 2121255
005 2021-05-24
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11033-020-05301-0$2DOI
100 1 $aPARAENSE, L. C. R.
245 $aFirst genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree.$h[electronic resource]
260 $c2020
500 $aShort Comunication.
520 $aPlatonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they were tested in 31 genotypes of P. insignis that belong to a germplasm bank and were sampled in the northeast of Pará State, Brazil. Thirteen primers were polymorphic and the number of alleles per loci varied from 5 (PI18 and PI27) to 2 (PI08, PI25, PI31, PI33 and PI 37). Expected heterozygosity (HE) varied from 0.74 (PI27) to 0.12 (PI31)b and observed heterozygosity (HO) varied from 1.00 (PI25) to 0.00 (PI08, PI31, PI33 and PI37). Principal coordinates could separate the genotypes of P. insignis in clusters and we can conclude that the primers can estimate the genetic diversity of P. insignis populations.
650 $aClusiaceae
650 $aOligonucleotides
650 $aBacuri
650 $aPlatonia Insignis
653 $aDiversidade genética
653 $aGenetic diversity
653 $aNext generation sequencing
653 $aSequenciamento genético
653 $aSimple sequence repeats
700 1 $aPENA, D. N.
700 1 $aDARNET, S. H.
700 1 $aRODRIGUES, S. de M.
700 1 $aMENEZES, I. C. de
700 1 $aCUNHA, E. F. M.
773 $tMolecular Biology Reports$gv. 47, n. 4, p. 2985-2989, fev. 2020.
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Embrapa Amazônia Oriental (CPATU) |
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