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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
Data corrente: |
17/12/2014 |
Data da última atualização: |
09/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL. |
Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, 2015. |
DOI: |
https://doi.org/10.1017/S0967199414000537 |
Idioma: |
Inglês |
Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
Thesaurus Nal: |
chromatin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1018303/1/Chemically-induced-enucleation-of-activated-bovine-oocytes.pdf
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Marc: |
LEADER 02607naa a2200277 a 4500 001 2018303 005 2024-02-09 008 2015 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/S0967199414000537$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource] 260 $c2015 520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. 650 $achromatin 653 $aBovine 653 $aChemically induced enucleation 653 $aMicrotubule 653 $aNuclear transfer 700 1 $aOLIVEIRA, C. S. 700 1 $aLEAL, C. L. V. 700 1 $aCALLADO, M. del 700 1 $aVANTINI, R. 700 1 $aMONTEIRO, F. M. 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tZygote$gv. 23, n. 6, p. 852-862, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
26/03/2015 |
Data da última atualização: |
17/04/2015 |
Tipo da produção científica: |
Circular Técnica |
Autoria: |
BRITO, L. G.; BARBIERI, F. da S.; ROCHA, R. B. |
Afiliação: |
LUCIANA GATTO BRITO, CPAF-RO; FABIO DA SILVA BARBIERI, CPAF-RO; RODRIGO BARROS ROCHA, CPAF-RO. |
Título: |
Fatores de risco associados à resistência à pesticidas em populações da mosca-dos-chifres. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Porto Velho: Embrapa Rondônia, 2014. |
Páginas: |
30 p. |
Série: |
(Embrapa Rondônia. Circular Técnica, 139). |
Idioma: |
Português |
Conteúdo: |
As pragas parasitárias são consideradas um dos principais fatores limitantes à produção animal em todo o mundo e, embora raramente associadas à alta mortalidade, determinam um expressivo impacto econômico em virtude da redução da produtividade e aumentos dos custos de controle. Essa situação tende a ser mais grave em regiões tropicais, como é o caso da maior parte do território brasileiro, onde as condições climáticas favoráveis contribuem para aumentar os problemas decorrentes do parasitismo. Esta publicação buscou levantamento os fatores de risco relacionados à resistência a pesticidas em rebanhos bovídeos. |
Palavras-Chave: |
Mosca-dos-chifres. |
Thesagro: |
Pesticida. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122468/1/CT-139-moscadoschifres.pdf
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Marc: |
LEADER 01202nam a2200181 a 4500 001 2012234 005 2015-04-17 008 2014 bl uuuu u0uu1 u #d 100 1 $aBRITO, L. G. 245 $aFatores de risco associados à resistência à pesticidas em populações da mosca-dos-chifres.$h[electronic resource] 260 $aPorto Velho: Embrapa Rondônia$c2014 300 $a30 p. 490 $a(Embrapa Rondônia. Circular Técnica, 139). 520 $aAs pragas parasitárias são consideradas um dos principais fatores limitantes à produção animal em todo o mundo e, embora raramente associadas à alta mortalidade, determinam um expressivo impacto econômico em virtude da redução da produtividade e aumentos dos custos de controle. Essa situação tende a ser mais grave em regiões tropicais, como é o caso da maior parte do território brasileiro, onde as condições climáticas favoráveis contribuem para aumentar os problemas decorrentes do parasitismo. Esta publicação buscou levantamento os fatores de risco relacionados à resistência a pesticidas em rebanhos bovídeos. 650 $aPesticida 653 $aMosca-dos-chifres 700 1 $aBARBIERI, F. da S. 700 1 $aROCHA, R. B.
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