Data/hora: 13/06/2024 18:38:41

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 04/11/2020
Data da última atualização: | 04/11/2020
Autoria: | ESPÍNDOLA, J. P.; BALBINOTT, N.; GRESSLER, L. T.; MACHADO, G.; KLEIN, C. S.; REBELATTO, R.; MARTÍN, C. B. G.; KREUTZ, L. C.; SCHRYVERS, A. B.; FRANDOLOSO, R.
Afiliação: | JÚLIA PIRES ESPÍNDOLA, UPF; NATÁLIA BALBINOTT, UPF; LETÍCIA TREVISAN GRESSLER, UPF; GUSTAVO MACHADO, North Carolina State University; CATIA SILENE KLEIN, CNPSA; RAQUEL REBELATTO, CNPSA; CÉSAR BERNARDO GUTIÉRREZ MARTÍN, University of León; LUIZ CARLOS KREUTZ, UPF; ANTHONY BERNARD SCHRYVERS, University of Calgary; RAFAEL FRANDOLOSO, UPF.
Título: | Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil.
Ano de publicação: | 2019
Fonte/Imprenta: | PeerJ, v. 7, n. e6817, 2019.
DOI: | http://doi.org/10.7717/peerj.6817
Idioma: | Inglês
Conteúdo: | Abstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. Disease mapping, Haemophilus parasuis, Typification, Serovars, Vaccines, Brazil Microbiology, Veterinary Medicine
Palavras-Chave: | Brasil; Disease mapping; Serovares; Serovars; Tipificação; Typification.
Thesagro: | Microbiologia; Vacina; Veterinária.
Thesaurus Nal: | Brazil; Haemophilus parasuis; Microbiology; Vaccines; Veterinary medicine.
Categoria do assunto: | --
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (21960-1) - UPC - AP - DD -  -  -  - DIS