Data/hora: 07/06/2024 16:19:09

Biblioteca(s): | Embrapa Tabuleiros Costeiros.
Data corrente: | 06/04/1998
Data da última atualização: | 06/04/1998
Autoria: | JONES, L. H.; HANKE, D. E.; EEWENS, C. J.
Título: | An evaluation of the role of cytokinins in the development of abnormal inflorescences in oil palms (Elaeis guineensis Jacq.) regenerated from tissue culture.
Ano de publicação: | 1995
Fonte/Imprenta: | Journal of Plant Growth Regulation, v. 14, n. 3, p. 135-142, 1995.
Idioma: | Inglês
Conteúdo: | Tissue cultures and regenerant plants from cell lines producing palms with normal and abnormal flowers were analysed for cytokinin content and compared with zygotic embryos and seedlings. Immature inflorescences at the critical stage of flower development dissected from normal and abnormal palms were also analysed. HPLC/radioimmunoassay and HPLC/enzyme-linked immunosorbent assay methods were used over a period of several years to measure the isoprenoid cytokinins. Oil palm cultures and regenerant plants contained relatively high concentrations of the 9-glucosides of isopentenyladenine ([9G]ip) and zeatin ([9g]Z). The predominant biologically active isoprenoid cytokinin present was zeatin riboside ([9R]Z), with lesser amounts of isopentenyladenine (iP) and isopentenyladenosine ([9R]iP). There was evidence of small amounts of dihydrozeatin compounds, but high concentrations (mainly as dihydrozeatin-9-glucoside ([9G]DHZ)) were confined to the haustorium of the zygotic embryo. Callus tissue contained very low concentrations of cytokinin. Comparison of 2 isogenic lines of one clone giving rise to normal and abnormal palms showed significantly higher concentrations of [9R]Z and [9G]Z in the normal than in the abnormal line and, in embryoids only, higher [9G]iP in the normal line. In all other cases the between-clone differences were greater than any normal/abnormal differences. There was a general tendency for increased concentrations of [9G]iP in abnormal lines and for this compound to be in a higher concentration in embryoids and plants derived from culture than in zygotic embryos and seedlings. Analysis of cytokinins in immature female inflorescences of normal and abnormal palms of a single clone showed the abnormal inflorescences to have higher concentrations of [9R]Z and [9R]DHZ and less [9G]Z than the normal inflorescences at comparable stages of development.
Palavras-Chave: | Oil palms; Plant embryos; Sementes; Tropical crops.
Thesagro: | Cultura de Tecido; Cultura In Vitro; Embrião Vegetal; Inflorescência; Palmeira Oleaginosa; Planta Oleaginosa; Regulador de Crescimento.
Thesaurus Nal: | cytokinins; growth regulators; in vitro culture; inflorescences; oil crops; oilseeds; physiology; plant growth substances; tissue culture; zeatin.
Categoria do assunto: | --
Registro original: | Embrapa Tabuleiros Costeiros (CPATC)
Exemplares: | CPATC - (14363-1) - ADD - SP - -- -  -  - SP001733 - DIS