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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/11/2004 |
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Data da última atualização: |
23/11/2015 |
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Autoria: |
CARVALHO, C. H. S.; ZEHR, U. B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H. H.; HODGES, T. K.; AXTELL, J. D. |
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Afiliação: |
CARLOS HENRIQUE S DE CARVALHO, Embrapa Milho e Sorgo; Usha B. Zehr, Purdue University; Nilupa Gunaratna, Purdue University; Joseph Anderson, Purdue University; Halina H. Kononowicz, Purdue University; Thomas K. Hodges, Purdue University; John D. Axtell, Purdue University. |
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Título: |
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 27, n. 2, p. 259-269, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection. MenosThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculu... Mostrar Tudo |
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Thesagro: |
Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32593/1/Agrobacterium-mediated.pdf
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Marc: |
LEADER 02225naa a2200205 a 4500
001 1488374
005 2015-11-23
008 2004 bl uuuu u00u1 u #d
100 1 $aCARVALHO, C. H. S.
245 $aAgrobacterium-mediated transformation of sorghum$bfactors that affect transformation efficiency.$h[electronic resource]
260 $c2004
520 $aThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection.
650 $aSorgo
700 1 $aZEHR, U. B.
700 1 $aGUNARATNA, N.
700 1 $aANDERSON, J.
700 1 $aKONONOWICZ, H. H.
700 1 $aHODGES, T. K.
700 1 $aAXTELL, J. D.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 27, n. 2, p. 259-269, 2004.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
27/11/2019 |
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Data da última atualização: |
06/10/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LIMA NETO, F. P.; LIMA, M. A. C. de; ISHIKAWA, F. H.; PARANHOS, J. G.; SOUZA, E. M. de C. |
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Afiliação: |
FRANCISCO PINHEIRO LIMA NETO, CPATSA; MARIA AUXILIADORA COELHO DE LIMA, CPATSA; FRANCINE HIROMI ISHIKAWA; JÉSSICA GIORDANO PARANHOS; EMILLE MAYARA DE CARVALHO SOUZA. |
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Título: |
Avaliação de híbridos de mangueira entre as variedades Tommy Atkins e Coquinho, em um ciclo, no Semiárido brasileiro. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 26., 2019, Juazeiro, BA/Petrolina, PE. Fruticultura de precisão: desafios e oportunidades - anais. Petrolina: Embrapa Semiárido: UNIVASF: SBF, 2019. |
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Páginas: |
p. 160-163. |
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Idioma: |
Português |
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Conteúdo: |
A diversificação de variedades é essencial para a consolidação das exportações brasileiras. O objetivo do trabalho consiste, assim, na avaliação de híbridos obtidos entre as variedades Tommy Atkins e Coquinho, em uma safra (2017-2018), no Semiárido brasileiro, considerando-se atributos físicos e químicos associados aos frutos. |
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Palavras-Chave: |
Atibutos físicos e químicos; Comportamento de variedades; Coquinho; Tommy Atkins. |
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Thesagro: |
Competição de Variedade; Manga. |
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Thesaurus NAL: |
Mangoes. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/205677/1/AVALIACAO-DE-HIBRIDOS-DE-MANGUEIRA.pdf
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Marc: |
LEADER 01275nam a2200253 a 4500
001 2115319
005 2023-10-06
008 2019 bl uuuu u00u1 u #d
100 1 $aLIMA NETO, F. P.
245 $aAvaliação de híbridos de mangueira entre as variedades Tommy Atkins e Coquinho, em um ciclo, no Semiárido brasileiro.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE FRUTICULTURA, 26., 2019, Juazeiro, BA/Petrolina, PE. Fruticultura de precisão: desafios e oportunidades - anais. Petrolina: Embrapa Semiárido: UNIVASF: SBF$c2019
300 $ap. 160-163.
520 $aA diversificação de variedades é essencial para a consolidação das exportações brasileiras. O objetivo do trabalho consiste, assim, na avaliação de híbridos obtidos entre as variedades Tommy Atkins e Coquinho, em uma safra (2017-2018), no Semiárido brasileiro, considerando-se atributos físicos e químicos associados aos frutos.
650 $aMangoes
650 $aCompetição de Variedade
650 $aManga
653 $aAtibutos físicos e químicos
653 $aComportamento de variedades
653 $aCoquinho
653 $aTommy Atkins
700 1 $aLIMA, M. A. C. de
700 1 $aISHIKAWA, F. H.
700 1 $aPARANHOS, J. G.
700 1 $aSOUZA, E. M. de C.
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Embrapa Semiárido (CPATSA) |
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