Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/08/2000 |
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Data da última atualização: |
09/01/2024 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
ALVES, F. S. F.; LEFEBVRE, R. B.; PROBERT, W. |
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Afiliação: |
FRANCISCO SELMO FERNANDES ALVES, CNPC; RANCE B. LEFEBVRE, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USA; WILLIAM PROBERT, University of California, Davis/Department of Veterinary Microbiology, Immunology and Pathology, 95616-Davis, CA, USA. |
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Título: |
Amino acid sequences of proteins from Leptospira serovar pomona. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, p. 95, n. 4, p. 503-504, jul./ago. 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstracts: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein. |
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Palavras-Chave: |
Sequence; Sequência. |
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Thesagro: |
Aminoácido; Doença animal; Leptospira pomona; Leptospirose; Proteína. |
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Thesaurus NAL: |
Amino acids; Animal diseases; Antigens; Leptospira; Proteins. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35400/1/API-Amino-acid.pdf
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Marc: |
LEADER 01513naa a2200289 a 4500
001 1515320
005 2024-01-09
008 2000 bl uuuu u00u1 u #d
100 1 $aALVES, F. S. F.
245 $aAmino acid sequences of proteins from Leptospira serovar pomona.$h[electronic resource]
260 $c2000
520 $aAbstracts: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein.
650 $aAmino acids
650 $aAnimal diseases
650 $aAntigens
650 $aLeptospira
650 $aProteins
650 $aAminoácido
650 $aDoença animal
650 $aLeptospira pomona
650 $aLeptospirose
650 $aProteína
653 $aSequence
653 $aSequência
700 1 $aLEFEBVRE, R. B.
700 1 $aPROBERT, W.
773 $tMemórias do Instituto Oswaldo Cruz, p. 95$gn. 4, p. 503-504, jul./ago. 2000.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
