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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
04/08/2016 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SALIMENA, A. P. S.; LANGE, C. C.; CAMUSSONE, C.; SIGNORINO, M.; CALVINHO, L. F.; BRITO, M. A. V. P. e; BORGES, C. A. V.; GUIMARAES, A. S.; RIBEIRO, J. B.; MENDONCA, L. C.; PICCOLI, R. H. |
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Afiliação: |
Alessandra P.S. Salimena, UFLA; CARLA CHRISTINE LANGE, CNPGL; Cecilia Camussone, INTA - Rafaela; Marcelo Signorini, INTA - Rafaela; Luis F. Calvinho, INTA - Rafaela; MARIA APARECIDA V PAIVA E BRITO, CNPGL; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; JOAO BATISTA RIBEIRO, CNPGL; LETICIA CALDAS MENDONCA, CNPGL; Roberta H. Piccoli, UFLA. |
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Título: |
Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Veterinary Research Communications, v. 40, n. 3/4, p. 97-106, 2016. |
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Páginas: |
10 p. |
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DOI: |
https://doi.org/10.1007/s11259-016-9658-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil. MenosStaphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer th... Mostrar Tudo |
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Palavras-Chave: |
Adhesin; Bap; Cap; Capsule; IcaAD. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02672naa a2200325 a 4500
001 2050220
005 2024-02-06
008 2016 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11259-016-9658-5$2DOI
100 1 $aSALIMENA, A. P. S.
245 $aGenotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms.$h[electronic resource]
260 $c2016
300 $a10 p.
520 $aStaphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
653 $aAdhesin
653 $aBap
653 $aCap
653 $aCapsule
653 $aIcaAD
700 1 $aLANGE, C. C.
700 1 $aCAMUSSONE, C.
700 1 $aSIGNORINO, M.
700 1 $aCALVINHO, L. F.
700 1 $aBRITO, M. A. V. P. e
700 1 $aBORGES, C. A. V.
700 1 $aGUIMARAES, A. S.
700 1 $aRIBEIRO, J. B.
700 1 $aMENDONCA, L. C.
700 1 $aPICCOLI, R. H.
773 $tVeterinary Research Communications$gv. 40, n. 3/4, p. 97-106, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 7 | |
| 2. |  | VELAME, D. R.; FERREIRA, C. F.; PEIXOUTO, Y. S.; RAMALHO, E. V. B. M.; SANTOS, V. da S.; OLIVEIRA, E. J. de. Diversidade genética de cultivares de mandioca com base em marcadores ISSR. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 3. | | RAMALHO, E. V. B. M.; VELAME, D. R.; PEIXOUTO, Y.; SANTOS, V. da S.; OLIVEIRA, E. J. de; FERREIRA, C. F. Diversidade genética entre cultivares de mandioca por meio de marcadores ISSR. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 6., 2012, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | VELAME, D. R.; PEIXOUTO, Y. S.; RAMALHO, E. V. B. M.; FERREIRA, C. F.; SILVA, R. A. da; SANTOS JÚNIOR, J. H. O. Uso de marcadores microssatélites na caracterização de variedades elite de mandioca (Manihot estulenta Crantz) em estudos de dialelos. In: REUNIÃO ANUAL DE CIÊNCIA, TECNOLOGIA, INOVAÇÃO E CULTURA NO RECÔNCAVO DA BAHIA - RECITEC RECÔNCAVO. 1., 2011, Cruz das Almas. Anais... Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2011. 1 CD-ROM. 2 p. Em paralelo aconteceram também os seguintes eventos: V Seminário de Pesquisa do Recôncavo da Bahia; V Seminário Estudantil de Pesquisa da UFRB; V Seminário da Pós-Graduação da UFRB; II Seminário Regional de Pesquisa da EBDA; 5ª Jornada...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | VELAME, D. R.; PEIXOUTO, Y. S.; RAMALHO, E. V. B. M.; SANTOS, V. da S.; OLIVEIRA, E. J. de; FERREIRA, C. F. Uso de marcadores SSR no estudo da diversidade genética entre cultivares de mandioca. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 6., 2012, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | PEIXOTO, Y. S.; CARNEIRO, J. L. dos S.; BASTOS, L. A.; VELAME, D. R.; SANTOS JÚNIOR, J. H. O.; RAMALHO, E. V. B. M.; OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de; SANTOS, V. da S.; FERREIRA, C. F. Diversidade genética entre parentais elite de mandioca como ferramenta para desenvolvimento de novas variedades. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 6., 2011, Búzios. Panorama atual e perspectivas do melhoramento de plantas no Brasil: [anais]. Búzios: Sociedade Brasileira de Melhoramento de Plantas, 2011. 1 CD ROM. 4 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | PEIXOUTO, Y. S.; BASTOS, L. A.; VELAME, D. R.; SANTOS JÚNIOR, J. H. O.; RAMALHO, E. V. B. M.; OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de; SANTOS, V. da S. Uso de marcadores microssatélites na caracterização de variedades elite de mandioca (Manihot esculenta Crantz) em estudos de dialelos. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 5., 2011, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2011. PDF. 093_11.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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