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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
02/06/2014 |
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Data da última atualização: |
23/09/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHICATTO, J. A.; CASTAMANN, V. A.; HELM, C. V.; TAVARES, L. B. B. |
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Afiliação: |
Juliane Andressa Chicatto, FURB; Vitória Arend Castamann, FURB; CRISTIANE VIEIRA HELM, CNPF; Lorena Benathar Ballod Tavares, FURB. |
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Título: |
Optimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Natural Resources, v. 5, n. 6, p. 241-255, Apr. 2014. |
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DOI: |
10.4236/nr.2014.56023 |
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Idioma: |
Inglês |
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Conteúdo: |
Issues such as fossil fuels and oil supplies have stimulated the search for renewable alternatives such as biofuels. Agricultural crop residues represent an abundant renewable resource for the future of bioethanol. For it to be a viable alternative, the second-generation ethanol which ought to provide a net energy gain, environmental benefits, should be economically viable, and also be produced in large quantities without reducing food supplies. The current difficulty of lignocellulosic biofuel production is the hydrolysis of biomass into sugar. This is a work in which the white-rot Lentinula edodes fungus secretes substantial amounts of hydrolytic enzymes and is useful for degradation of lignocellulosic biomass which have not been described yet. The objective of this investigation was to evaluate the pH effect (5, 6 and 7), agitation (0, 100 rpm and 200 rpm) and also the cultivation time (6, 9 and 12 days). The culture medium was supplemented with agro-industrial residue and the EF 52 strain of the fungus Lentinula edodes was used as a processing agent. A factorial design 22 repeating the central point was performed. Submerged cultivation was conducted in a synthetic medium and was incubated at 25?C. The total protein content was determined as well as the activity of xylanase and cellulase (endoglucanase, exoglucanase and ?-glucosidase). By Pareto diagram, the agitation and pH variables were significant for enzymatic activities. The highest enzyme expression occurred at pH values between 5.0 and 6.0 and above 100 rpm agitation. The exoglucanase was the enzyme which showed the highest activity in terms of cellulases, despite the cultivation time. Regarding the production of other enzymes and proteins, the most significant cultivation time was 12 days. MenosIssues such as fossil fuels and oil supplies have stimulated the search for renewable alternatives such as biofuels. Agricultural crop residues represent an abundant renewable resource for the future of bioethanol. For it to be a viable alternative, the second-generation ethanol which ought to provide a net energy gain, environmental benefits, should be economically viable, and also be produced in large quantities without reducing food supplies. The current difficulty of lignocellulosic biofuel production is the hydrolysis of biomass into sugar. This is a work in which the white-rot Lentinula edodes fungus secretes substantial amounts of hydrolytic enzymes and is useful for degradation of lignocellulosic biomass which have not been described yet. The objective of this investigation was to evaluate the pH effect (5, 6 and 7), agitation (0, 100 rpm and 200 rpm) and also the cultivation time (6, 9 and 12 days). The culture medium was supplemented with agro-industrial residue and the EF 52 strain of the fungus Lentinula edodes was used as a processing agent. A factorial design 22 repeating the central point was performed. Submerged cultivation was conducted in a synthetic medium and was incubated at 25?C. The total protein content was determined as well as the activity of xylanase and cellulase (endoglucanase, exoglucanase and ?-glucosidase). By Pareto diagram, the agitation and pH variables were significant for enzymatic activities. The highest enzyme expression occurred at pH ... Mostrar Tudo |
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Palavras-Chave: |
Basidiomiceto; Basidiomycetes; Bioetanol; Cellulase; Hidrólise enzimática; Xilanase; Xylanase. |
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Thesagro: |
Celulase. |
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Thesaurus Nal: |
bioethanol; enzymatic hydrolysis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102997/1/2014-API-CrisH-OptimizationProduction.pdf
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Marc: |
LEADER 02625naa a2200289 a 4500
001 1987490
005 2016-09-23
008 2014 bl uuuu u00u1 u #d
024 7 $a10.4236/nr.2014.56023$2DOI
100 1 $aCHICATTO, J. A.
245 $aOptimization of the production process of enzymatic activity of Lentinula edodes (Berk.) Pegler in holocelulases.$h[electronic resource]
260 $c2014
520 $aIssues such as fossil fuels and oil supplies have stimulated the search for renewable alternatives such as biofuels. Agricultural crop residues represent an abundant renewable resource for the future of bioethanol. For it to be a viable alternative, the second-generation ethanol which ought to provide a net energy gain, environmental benefits, should be economically viable, and also be produced in large quantities without reducing food supplies. The current difficulty of lignocellulosic biofuel production is the hydrolysis of biomass into sugar. This is a work in which the white-rot Lentinula edodes fungus secretes substantial amounts of hydrolytic enzymes and is useful for degradation of lignocellulosic biomass which have not been described yet. The objective of this investigation was to evaluate the pH effect (5, 6 and 7), agitation (0, 100 rpm and 200 rpm) and also the cultivation time (6, 9 and 12 days). The culture medium was supplemented with agro-industrial residue and the EF 52 strain of the fungus Lentinula edodes was used as a processing agent. A factorial design 22 repeating the central point was performed. Submerged cultivation was conducted in a synthetic medium and was incubated at 25?C. The total protein content was determined as well as the activity of xylanase and cellulase (endoglucanase, exoglucanase and ?-glucosidase). By Pareto diagram, the agitation and pH variables were significant for enzymatic activities. The highest enzyme expression occurred at pH values between 5.0 and 6.0 and above 100 rpm agitation. The exoglucanase was the enzyme which showed the highest activity in terms of cellulases, despite the cultivation time. Regarding the production of other enzymes and proteins, the most significant cultivation time was 12 days.
650 $abioethanol
650 $aenzymatic hydrolysis
650 $aCelulase
653 $aBasidiomiceto
653 $aBasidiomycetes
653 $aBioetanol
653 $aCellulase
653 $aHidrólise enzimática
653 $aXilanase
653 $aXylanase
700 1 $aCASTAMANN, V. A.
700 1 $aHELM, C. V.
700 1 $aTAVARES, L. B. B.
773 $tNatural Resources$gv. 5, n. 6, p. 241-255, Apr. 2014.
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Registro original: |
Embrapa Florestas (CNPF) |
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| Registros recuperados : 6 | |
| 5. |  | SILVA, P. H. M. da; PAULA, R. C. de; MIRANDA, A. C.; MORAES, M. L. T. de; FURTADO, E. L.; PIERI, C. de; BRAWNER, J.; LEE, D. Screening of rust and gall wasp in eucalypts species and provenances. Pesquisa Florestal Brasileira, Colombo, v. 35, n. 83, p. 323-328, jul./set. 2015. Nota científica.| Biblioteca(s): Embrapa Florestas. |
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| 6. | | OLIVEIRA, T. W. G. de; PAULA, R. C. de; MORAES, M. L. T. de; ALVARES, C. A.; MIRANDA, A. C.; SILVA, P. H. M. da. Stability and adaptability for wood volume in the selection of Eucalyptus saligna in three environments. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 5, p. 611-619, maio, 2018. Título em português: Estabilidade e adaptabilidade para volume de madeira na seleção de Eucalyptus saligna em três ambientes.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 6 | |
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