Data/hora: 23/09/2024 00:46:33

Biblioteca(s): 	Embrapa Agricultura Digital; Embrapa Florestas.	
Data corrente: 	13/11/2019	
Data da última atualização: 	13/03/2020	
Autoria: 	BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J.	
Afiliação: 	Gisela Manuela de França Bettencourt, UFPR; Carlos Ricardo Soccol, UFPR; Thais Salete Giovanella, UFPR; LUZIANE FRANCISCON, CNPF; DAIANE RIGONI, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JULIANA DEGENHARDT GOLDBACH, CNPF.	
Título: 	Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020.	
DOI: 	10.1007/s11676-018-0777-4	
Idioma: 	Inglês	
Conteúdo: 	Abstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated.	
Palavras-Chave: 	Plant growth regulators; Regeneração in vitro; Reguladores de crescimento de plantas; Transformação genética.	
Thesagro: 	Eucalipto; Eucalyptus Urophylla.	
Thesaurus Nal: 	Genetic transformation; In vitro regeneration; Somaclonal variation.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Florestas (CNPF)	
Exemplares: 	CNPF - (57074-1) - UPC - AP - DD -  -  -  - DIS
CNPTIA - (20136-1) - UPC - AP - DD -  -  -  - DIS