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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/08/1999 |
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Data da última atualização: |
16/05/2023 |
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Autoria: |
SCHOPKE, C.; TAYLOR, N. J.; CARCAMO, R.; BEACHY, R. N.; FAUQUET, C. |
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Afiliação: |
International Laboratory for Tropical Agricultural Biotechnology. The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. |
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Título: |
Optimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Plant Cell Reports, v.16, p.526-530, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Tissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uidA gene, which codes for glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. A reproducible counting method was established by optimizing GUS assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue, The effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on GUS expression were investigated. |
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Palavras-Chave: |
Embryogenic suspension cultures; Ferricyanide; Ferrocyanide; Glucuronidase; Microparticle bombardment. |
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Thesaurus Nal: |
image analysis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01632naa a2200241 a 4500
001 1648243
005 2023-05-16
008 1997 bl uuuu u00u1 u #d
100 1 $aSCHOPKE, C.
245 $aOptimization of parameters for particle bombardment of embryogenic suspension cultures of cassava (Manihot esculenta Crantz) using computer image analysis.$h[electronic resource]
260 $c1997
520 $aTissue derived from embryogenic suspension cultures of cassava was bombarded with microparticles coated with a plasmid containing the uidA gene, which codes for glucuronidase (GUS). After 3 days, the effect of different bombardment parameters was evaluated by comparing the numbers of blue spots that resulted from histological GUS assays. Counting of blue spots was performed using a system comprised of a black and white video camera, a stereoscope and a personal computer. A reproducible counting method was established by optimizing GUS assay conditions, preparation of tissue samples and acquisition of video images in view of attaining the highest possible contrast between the blue spots and the surrounding tissue, The effects of bombardment pressure, microparticle size, number of bombardments, and osmotic pretreatment on GUS expression were investigated.
650 $aimage analysis
653 $aEmbryogenic suspension cultures
653 $aFerricyanide
653 $aFerrocyanide
653 $aGlucuronidase
653 $aMicroparticle bombardment
700 1 $aTAYLOR, N. J.
700 1 $aCARCAMO, R.
700 1 $aBEACHY, R. N.
700 1 $aFAUQUET, C.
773 $tPlant Cell Reports$gv.16, p.526-530, 1997.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
23/09/2004 |
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Data da última atualização: |
01/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
CARBONEL, S. A. M.; KRZYZANOWSKI, F. C.; MESQUITA, C. M. |
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Afiliação: |
FRANCISCO CARLOS KRZYZANOWSKI, CNPSO. |
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Título: |
A device to impart impact on soybean seeds for screening genotypes for resistance to mechanical damage. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Seed Science and Technology, Zurich, v. 26, n. 1, p. 45-52, 1998. |
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Idioma: |
Inglês |
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Thesagro: |
Dano Mecânico; Semente; Soja. |
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Thesaurus NAL: |
Mechanical damage; Seeds; Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 00628naa a2200205 a 4500
001 1466911
005 2024-02-01
008 1998 bl uuuu u00u1 u #d
100 1 $aCARBONEL, S. A. M.
245 $aA device to impart impact on soybean seeds for screening genotypes for resistance to mechanical damage.$h[electronic resource]
260 $c1998
650 $aMechanical damage
650 $aSeeds
650 $aSoybeans
650 $aDano Mecânico
650 $aSemente
650 $aSoja
700 1 $aKRZYZANOWSKI, F. C.
700 1 $aMESQUITA, C. M.
773 $tSeed Science and Technology, Zurich$gv. 26, n. 1, p. 45-52, 1998.
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Embrapa Soja (CNPSO) |
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