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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
11/05/2023 |
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Autoria: |
HEBERT, G. A.; PELHAM, P. L.; PITTMAN, B. |
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Título: |
Determination of the optimol ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. |
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Ano de publicação: |
1973 |
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Fonte/Imprenta: |
Applied Microbiology, v. 25, n. 1, p. 23-36, Jan. 1973. |
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DOI: |
DOI: 10.1128/am.25.1.26-36.1973 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Various ammonium sulfate concentrations and reaction conditions were employed in the fractionation of sera from rabbits, sheep, horses, and goats. Precipitates and supernatant fluids were analyzed by electrophoresis to study the effects of the controlled variables. At room temperature, the third precipitate in 35% saturated (NH)2SO4 was the best fraction from both rabbit and sheep sera; 80 to 90% of the gamma globulins were recovered. The second and third precipitates of horse sera proteins in 30% saturated (NH),2SO0 were both satisfactory, but only 44% of the gamma globulin was recovered after three precipitations. Goat sera yielded a very satisfactory fraction; 80% of the gamma globulin was recovered after two precipitations-the first in 30% and the second in 45% saturated (NH,)2SO. The composition of these fractions was not influenced by the pH of the sulfate solutions (pH 5.8 and 7.2), by a range of normal room temperatures (20 to 30 C), or by diluting the sera before fractionation. Crude globulins and fluorescein isothiocyanate-labeled globulins were successfully refractionated by one precipitation in the optimal sulfate concentration for the appropriate animal species. The refractionated products contained considerably less beta and alpha globulins than did the original crude fractions and little or no albumin. |
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Palavras-Chave: |
Fluoresceins; Hydrogen ion concentration; Immune Sera; Spectrophotometry. |
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Thesagro: |
Caprino; Cavalo; Coelho; Imunologia; Ovino. |
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Thesaurus Nal: |
Alpha-globulins; Ammonium sulfate; Bacterial antigens; Blood proteins; Electrophoresis; Gamma-globulins; Goats; Horses; Immunology; Quaternary ammonium compounds; Rabbits; Serum albumin; Sheep; Temperature. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02556naa a2200433 a 4500
001 1520486
005 2023-05-11
008 1973 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1128/am.25.1.26-36.1973$2DOI
100 1 $aHEBERT, G. A.
245 $aDetermination of the optimol ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera.$h[electronic resource]
260 $c1973
520 $aAbstract: Various ammonium sulfate concentrations and reaction conditions were employed in the fractionation of sera from rabbits, sheep, horses, and goats. Precipitates and supernatant fluids were analyzed by electrophoresis to study the effects of the controlled variables. At room temperature, the third precipitate in 35% saturated (NH)2SO4 was the best fraction from both rabbit and sheep sera; 80 to 90% of the gamma globulins were recovered. The second and third precipitates of horse sera proteins in 30% saturated (NH),2SO0 were both satisfactory, but only 44% of the gamma globulin was recovered after three precipitations. Goat sera yielded a very satisfactory fraction; 80% of the gamma globulin was recovered after two precipitations-the first in 30% and the second in 45% saturated (NH,)2SO. The composition of these fractions was not influenced by the pH of the sulfate solutions (pH 5.8 and 7.2), by a range of normal room temperatures (20 to 30 C), or by diluting the sera before fractionation. Crude globulins and fluorescein isothiocyanate-labeled globulins were successfully refractionated by one precipitation in the optimal sulfate concentration for the appropriate animal species. The refractionated products contained considerably less beta and alpha globulins than did the original crude fractions and little or no albumin.
650 $aAlpha-globulins
650 $aAmmonium sulfate
650 $aBacterial antigens
650 $aBlood proteins
650 $aElectrophoresis
650 $aGamma-globulins
650 $aGoats
650 $aHorses
650 $aImmunology
650 $aQuaternary ammonium compounds
650 $aRabbits
650 $aSerum albumin
650 $aSheep
650 $aTemperature
650 $aCaprino
650 $aCavalo
650 $aCoelho
650 $aImunologia
650 $aOvino
653 $aFluoresceins
653 $aHydrogen ion concentration
653 $aImmune Sera
653 $aSpectrophotometry
700 1 $aPELHAM, P. L.
700 1 $aPITTMAN, B.
773 $tApplied Microbiology$gv. 25, n. 1, p. 23-36, Jan. 1973.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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