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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
12/08/2021 |
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Data da última atualização: |
12/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, Y. D.; PEREIRA, W. A.; PAULA, C. M. P. de; RUME, G. de C.; LIMA, A. A.; CHALFUN-JUNIOR, A.; SOUZA SOBRINHO, F. de; TECHIO, V. H. |
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Afiliação: |
YASMIM DUTRA SANTOS, Universidade Federal de Lavras; WELISON ANDRADE PEREIRA, Universidade Federal de Lavras; CRISTINA MARIA PINTO DE PAULA, Universidade Federal de Lavras; GABRIEL DE CAMPOS RUME, Universidade Federal de Lavras; ANDRÉ ALMEIDA LIMA, Universidade Federal de Lavras; ANTONIO CHALFUN-JUNIOR, Universidade Federal de Lavras; FAUSTO DE SOUZA SOBRINHO, CNPGL; VÂNIA HELENA TECHIO, Universidade Federal de Lavras. |
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Título: |
Epigenetic marks associated to the study of nucleolar dominance in Urochloa P. Beauv. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 38, p. 380-393, 2020. |
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DOI: |
https://doi.org/10.1007/s11105-020-01203-4 |
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Idioma: |
Inglês |
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Conteúdo: |
The genus Urochloa P. Beauv. presents a prominent role in the tropical agricultural scenario being composed of species with different ploidy levels. Studies on the genomic relationship within this genus as well as specific analysis involving epigenetic marks are limited. The aim of the present study was to identify the cytosine methylation (5-mCyt) and histone H3 lysine 9 dimethylation (H3k9me2) in the different modulations of 45S ribosomal DNA (rDNA) sites in interphase nuclei and to associate these results with gene expression analysis in Urochloa ruziziensis (2n = 4x = 36), Urochloa brizantha cv. Marandu (2n = 4x = 36), and their respective interspecific hybrid H1863 (2n = 4x = 36). Immunolocalization techniques were performed in combination with Fluorescence in situ hybridization (FISH) for the location of the 45S rDNA sites. Predominantly, we observed intraand perinucleolar sites, mostly hypomethylated and/or hyper/hypomethylated, decondensed or partially condensed. The gene expression analysis was performed qualitatively through the conventional PCR using complementary DNA and confirmed by the RT-qPCR technique and primers designed for the ITS-1 region of U. brizantha and U. ruziziensis. The molecular analyses performed on leaves showed that there is dominance of U. brizantha 45S rDNA gene expression on U. ruziziensis in the H1863 hybrid. In roots, the analyses showed that the 45S rDNA genes of the two parents are expressed in the hybrid genome. Thus, it is plausible to infer a tissue-specific nuclear dominance model in which the pattern of hypermethylated cytosine sites with heterochromatic marks and, therefore, silenced were mostly inherited from U. ruziziensis, whereas the rDNA originated from U. brizantha was characterized by cytosine and H3k9 hypomethylation. MenosThe genus Urochloa P. Beauv. presents a prominent role in the tropical agricultural scenario being composed of species with different ploidy levels. Studies on the genomic relationship within this genus as well as specific analysis involving epigenetic marks are limited. The aim of the present study was to identify the cytosine methylation (5-mCyt) and histone H3 lysine 9 dimethylation (H3k9me2) in the different modulations of 45S ribosomal DNA (rDNA) sites in interphase nuclei and to associate these results with gene expression analysis in Urochloa ruziziensis (2n = 4x = 36), Urochloa brizantha cv. Marandu (2n = 4x = 36), and their respective interspecific hybrid H1863 (2n = 4x = 36). Immunolocalization techniques were performed in combination with Fluorescence in situ hybridization (FISH) for the location of the 45S rDNA sites. Predominantly, we observed intraand perinucleolar sites, mostly hypomethylated and/or hyper/hypomethylated, decondensed or partially condensed. The gene expression analysis was performed qualitatively through the conventional PCR using complementary DNA and confirmed by the RT-qPCR technique and primers designed for the ITS-1 region of U. brizantha and U. ruziziensis. The molecular analyses performed on leaves showed that there is dominance of U. brizantha 45S rDNA gene expression on U. ruziziensis in the H1863 hybrid. In roots, the analyses showed that the 45S rDNA genes of the two parents are expressed in the hybrid genome. Thus, it is plausible t... Mostrar Tudo |
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Palavras-Chave: |
Citosina; Dimetilação; DNA ribossomal; Expressão gênica; Histona; Nucleolar dominance. |
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Thesagro: |
Brachiaria; Metilação. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
null
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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