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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
11/03/2021 |
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Data da última atualização: |
11/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRIGUEZ YON, Y.; MAISTRO PATREZE, C.; SAGGIN JUNIOR, O. J.; ANTONIO RIVERA, R.; QUINONES, M.; HAESAERT, G.; VAN TUINEN, D. |
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Afiliação: |
YAKELIN RODRÍGUEZ YON, INSTITUTO DE CIÊNCIA AGRICOLA, CUBA; UNIRIO; ORIVALDO JOSE SAGGIN JUNIOR, CNPAB; INSTITUTO DE CIÊNCIA AGRICOLA, CUBA; MADELAINE QUIÑONES, Centro Nacional de Sanidad Agropecuaria, CUBA; GEERT HAESAERT, Ghent University; DIEDERIK VAN TUINEN, Université Bourgogne Franche-Comté. |
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Título: |
Development of a taxon discriminating molecular marker to trace and quantify a mycorrhizal inoculum in roots and soils of agroecosystems. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Folia Microbiologica, published online: 03 february 2021. |
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DOI: |
https://doi.org/10.1007/s12223-020-00844- |
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Idioma: |
Inglês |
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Conteúdo: |
Crop inoculation with Glomus cubense isolate (INCAM-4, DAOM-241198) promotes yield in banana, cassava, forages, and others. Yield improvements range from 20 to 80% depending on crops, nutrient supply, and edaphoclimatic conditions. However, it is difcult to connect yield efects with G. cubense abundance in roots due to the lack of an adequate methodology to trace this taxon in the feld. It is necessary to establish an accurate evaluation framework of its contribution to root colonization separated from native arbuscular mycorrhizal fungi (AMF). A taxon-discriminating primer set was designed based on the ITS nrDNA marker and two molecular approaches were optimized and validated (endpoint PCR and quantitative real-time PCR) to trace and quantify the G. cubense isolate in root and soil samples under greenhouse and environmental conditions. The detection limit and specifcity assays were performed by both approaches. Diferent 18 AMF taxa were used for endpoint PCR specifcity assay, showing that primers specifcally amplifed the INCAM-4 isolate yielding a 370 bp-PCR product. In the greenhouse, Urochloa brizantha plants inoculated with three isolates (Rhizophagus irregularis, R. clarus, and G. cubense) and environmental root and soil samples were successfully traced and quantifed by qPCR. The AMF root colonization reached 41?70% and the spore number 4?128 per g of soil. This study demonstrates for the frst time the feasibility to trace and quantify the G. cubense isolate using a taxon-discriminating ITS marker in roots and soils. The validated approaches reveal their potential to be used for the quality control of other mycorrhizal inoculants and their relative quantifcation in agroecosyst. MenosCrop inoculation with Glomus cubense isolate (INCAM-4, DAOM-241198) promotes yield in banana, cassava, forages, and others. Yield improvements range from 20 to 80% depending on crops, nutrient supply, and edaphoclimatic conditions. However, it is difcult to connect yield efects with G. cubense abundance in roots due to the lack of an adequate methodology to trace this taxon in the feld. It is necessary to establish an accurate evaluation framework of its contribution to root colonization separated from native arbuscular mycorrhizal fungi (AMF). A taxon-discriminating primer set was designed based on the ITS nrDNA marker and two molecular approaches were optimized and validated (endpoint PCR and quantitative real-time PCR) to trace and quantify the G. cubense isolate in root and soil samples under greenhouse and environmental conditions. The detection limit and specifcity assays were performed by both approaches. Diferent 18 AMF taxa were used for endpoint PCR specifcity assay, showing that primers specifcally amplifed the INCAM-4 isolate yielding a 370 bp-PCR product. In the greenhouse, Urochloa brizantha plants inoculated with three isolates (Rhizophagus irregularis, R. clarus, and G. cubense) and environmental root and soil samples were successfully traced and quantifed by qPCR. The AMF root colonization reached 41?70% and the spore number 4?128 per g of soil. This study demonstrates for the frst time the feasibility to trace and quantify the G. cubense isolate using a tax... Mostrar Tudo |
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Palavras-Chave: |
Arbuscular mycorrhizal fungi; Mycorrhizal inoculant. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02499naa a2200229 a 4500
001 2130609
005 2021-03-11
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12223-020-00844-$2DOI
100 1 $aRODRIGUEZ YON, Y.
245 $aDevelopment of a taxon discriminating molecular marker to trace and quantify a mycorrhizal inoculum in roots and soils of agroecosystems.$h[electronic resource]
260 $c2021
520 $aCrop inoculation with Glomus cubense isolate (INCAM-4, DAOM-241198) promotes yield in banana, cassava, forages, and others. Yield improvements range from 20 to 80% depending on crops, nutrient supply, and edaphoclimatic conditions. However, it is difcult to connect yield efects with G. cubense abundance in roots due to the lack of an adequate methodology to trace this taxon in the feld. It is necessary to establish an accurate evaluation framework of its contribution to root colonization separated from native arbuscular mycorrhizal fungi (AMF). A taxon-discriminating primer set was designed based on the ITS nrDNA marker and two molecular approaches were optimized and validated (endpoint PCR and quantitative real-time PCR) to trace and quantify the G. cubense isolate in root and soil samples under greenhouse and environmental conditions. The detection limit and specifcity assays were performed by both approaches. Diferent 18 AMF taxa were used for endpoint PCR specifcity assay, showing that primers specifcally amplifed the INCAM-4 isolate yielding a 370 bp-PCR product. In the greenhouse, Urochloa brizantha plants inoculated with three isolates (Rhizophagus irregularis, R. clarus, and G. cubense) and environmental root and soil samples were successfully traced and quantifed by qPCR. The AMF root colonization reached 41?70% and the spore number 4?128 per g of soil. This study demonstrates for the frst time the feasibility to trace and quantify the G. cubense isolate using a taxon-discriminating ITS marker in roots and soils. The validated approaches reveal their potential to be used for the quality control of other mycorrhizal inoculants and their relative quantifcation in agroecosyst.
653 $aArbuscular mycorrhizal fungi
653 $aMycorrhizal inoculant
700 1 $aMAISTRO PATREZE, C.
700 1 $aSAGGIN JUNIOR, O. J.
700 1 $aANTONIO RIVERA, R.
700 1 $aQUINONES, M.
700 1 $aHAESAERT, G.
700 1 $aVAN TUINEN, D.
773 $tFolia Microbiologica, published online: 03 february 2021.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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