Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
17/02/2020 |
Data da última atualização: |
23/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MACHADO, J. S.; DEGENHARDT, J.; MAIA, F. R.; QUOIRIN, M. |
Afiliação: |
Jean Santos Machado, Programa de Pós-Graduação em Botânica, UFPR; JULIANA DEGENHARDT, CNPF; Fabiano Rodrigo Maia, Programa de Pós-Graduação em Botânica, UFPR; Marguerite Quoirin, Programa de Pós-Graduação em Botânica, UFPR. |
Título: |
Micropropagation of Campomanesia xanthocarpa O. Berg (Myrtaceae), a medicinal tree from the Brazilian Atlantic Forest. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Trees, v. 34, n. 3, p. 791-799, June 2020. |
DOI: |
https://doi.org/10.1007/s00468-020-01958-z |
Idioma: |
Inglês |
Conteúdo: |
Campomanesia xanthocarpa O. Berg (Myrtaceae), popularly known as guabiroba, is a woody species native to Brazil, important for its potential as a medicinal plant and fruit tree. For the frst time, this study shows a complete system of micropropagation for this species. For this, nodal segments with two axillary buds each were used as starting material. Plant Preservative Mixture? (0.1%) added to culture media was efcient in controlling contamination throughout the culture process. Two formulations of culture medium were compared for in vitro establishment. 2-Isopentenyladenine, 6-benzyladenine, kinetin and zeatin were tested during the multiplication step. Woody plant medium (WPM) was appropriate for all culture steps and, during the multiplication process, 2.2 µM BA induced the best numbers of new shoots per explant during three subcultures (3.0?3.5 per month). For rooting of microcuttings, a rate of 53% was reached in the WPM supplemented with 4.9 µM indol-3-butyric acid. A plastic box containing a mixture of commercial substrate and vermiculite (1:1 v/v) was used for plantlet acclimatization, allowing 52% of survival after two months. In conclusion, a complete micropropagation protocol was developed providing healthy plants. Further studies are needed to improve multiplication and survival rates. |
Palavras-Chave: |
Plant preservative mixture; Woody plant medium. |
Thesagro: |
Espécie Nativa; Guabiroba; Micropropagação. |
Thesaurus Nal: |
Campomanesia xanthocarpa; Cytokinins; Micropropagation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02152naa a2200265 a 4500 001 2120344 005 2020-11-23 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s00468-020-01958-z$2DOI 100 1 $aMACHADO, J. S. 245 $aMicropropagation of Campomanesia xanthocarpa O. Berg (Myrtaceae), a medicinal tree from the Brazilian Atlantic Forest.$h[electronic resource] 260 $c2020 520 $aCampomanesia xanthocarpa O. Berg (Myrtaceae), popularly known as guabiroba, is a woody species native to Brazil, important for its potential as a medicinal plant and fruit tree. For the frst time, this study shows a complete system of micropropagation for this species. For this, nodal segments with two axillary buds each were used as starting material. Plant Preservative Mixture? (0.1%) added to culture media was efcient in controlling contamination throughout the culture process. Two formulations of culture medium were compared for in vitro establishment. 2-Isopentenyladenine, 6-benzyladenine, kinetin and zeatin were tested during the multiplication step. Woody plant medium (WPM) was appropriate for all culture steps and, during the multiplication process, 2.2 µM BA induced the best numbers of new shoots per explant during three subcultures (3.0?3.5 per month). For rooting of microcuttings, a rate of 53% was reached in the WPM supplemented with 4.9 µM indol-3-butyric acid. A plastic box containing a mixture of commercial substrate and vermiculite (1:1 v/v) was used for plantlet acclimatization, allowing 52% of survival after two months. In conclusion, a complete micropropagation protocol was developed providing healthy plants. Further studies are needed to improve multiplication and survival rates. 650 $aCampomanesia xanthocarpa 650 $aCytokinins 650 $aMicropropagation 650 $aEspécie Nativa 650 $aGuabiroba 650 $aMicropropagação 653 $aPlant preservative mixture 653 $aWoody plant medium 700 1 $aDEGENHARDT, J. 700 1 $aMAIA, F. R. 700 1 $aQUOIRIN, M. 773 $tTrees$gv. 34, n. 3, p. 791-799, June 2020.
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Registro original: |
Embrapa Florestas (CNPF) |
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