02115naa a2200229 a 450000100080000000500110000800800410001902400320006010000220009224501570011426000090027152013390028065300250161970000190164470000230166370000240168670000170171070000190172770000250174670000160177177300980178719965232017-08-29       2014    bl uuuu     u00u1 u    #d7 a10.1099/ijs.0.064543-02DOI1 aDALL'AGNOL, R. F.  aRhizobium paranaense sp. nov., an effective N2 - fixing symbiont of common bean (Phaseolus vulgaris L.) with broad greographical distribution in Brazil.  c2014  aNitrogen (N), the nutrient most required for plant growth, is key for good yield of agriculturally important crops. Common bean (Phaseolus vulgaris L.) can benefit from bacteria collectively called rhizobia, which are capable of fixing atmospheric nitrogen (N2) in root nodules and supplying it to the plant. Common bean is amongst the most promiscuous legume hosts; several described species, in addition to putative new ones have been reported as able to nodulate this legume, although not always effectively in terms of fixing N2. Here we present data indicating that Brazilian strains PRF 35T, PRF 54, CPAO 1135 and H 52-currently classified as Rhizobium tropici-represent a new species symbiont of common bean. Morphological, physiological and biochemical properties differentiate these stains from other Rhizobium species, as do BOX-PCR profiles (less than 60% similarity), multilocus sequence analysis with recA, gyrB and rpoA genes (less than 96.4% identity), DNA-DNA hybridization (less than 50% DNA relatedness), and ANI of whole genomes (less than 92.8.%). The novel species is effective in nodulating and fixing N2 with P. vulgaris, Leucaena leucocephala and Leucaena esculenta. We propose the name Rhizobium paranaense sp. nov. for this new taxon, and PRF 35T (=CNPSo 120T, =LMG 27577T, =IPR-Pv 1249T) as the type strain.  aRhizobium paranaense1 aRIBEIRO, R. A.1 aDELAMUTA, J. R. M.1 aORMEÑO-ORRILLO, E.1 aROGEL, M. A.1 aANDRADE, D. S.1 aMARTÍNEZ-ROMERO, E.1 aHUNGRIA, M.  tInternational Journal of Systematic and Evolutionary Microbiologygv. 64, p. 3222-3229, 2014.