03085naa a2200229 a 450000100080000000500110000800800410001910000140006024501540007426000090022852023490023765000230258665300210260965300290263065300120265965300330267170000200270470000190272470000220274370000200276577300700278519822622014-03-13       2013    bl uuuu     u00u1 u    #d1 aDIOLA, V.  aA new set of differentially expressed signaling genes is early expressed in coffee leaf rust race II incompatible interaction.h[electronic resource]  c2013  aNew races of coffee rust are overcoming resistance genes available in germplasm and cultivated cultivars and bringing recently some coffee-producing countries in severe economic challenge. The objective of this study was to identify the genes that are linked to host resistance to the major coffee rust race II. In our study, we have identified and studied a segregating population that has a single monogenic resistant gene to coffee rust. Coffee leaves of parents, resistant, and susceptible genotypes of the F2 generation plants were inoculated with pathogen spores. A differential analysis was performed by combined cDNA-AFLP and bulk segregant analysis (BSA) in pooled samples collected 48 and 72 h postinoculation, increasing the selectiveness for differential gene expression. Of 108 differential expressed genes, between 33,000 gene fragments analyzed, 108 differential expressed genes were identified in resistant plants. About 20 and 22 % of these resistant-correlated genes are related to signaling and defense genes, respectively. Between signaling genes, the major subclass corresponds to receptor and resistant homolog genes, like nucleotide-binding site leucine-rich repeat (NBSLRR), Pto-like, RLKs, Bger, and RGH1A, all not previously described in coffee rust responses. The second major subclass included kinases, where two mitogen-activated kinases (MAPK) are identified. Further gene expression analysis was performed for 21 selected genes by real-time PCR gene expression analysis at 0, 12, 24, 48, and 72 h postinoculation. The expression of genes involved in signaling and defense was higher at 24 and 72 h after inoculation, respectively. The NBS-LRR was the more differentially expressed gene between the signaling genes (four times more expressed in the resistant genotype), and thraumatin (PR5) was the more expressed between all genes (six times more expressed). Multivariate analysis reinforces the significance of the temporal separation of identified signaling and defense genes: early expression of signaling genes support the hypothesis that higher expression of the signaling components up regulates the defense genes identified. Additionally the increased gene expression of these two gene sets is associated with a single monogenic resistance trait to to leaf coffee rust in the nteraction characterized here.  aHemileia Vastatrix  aDefense response  aHost-specific resistance  aR-genes  aTranscript-derived fragments1 aBRITO, G. G. de1 aCAIXETA, E. T.1 aPEREIRA, L. F. P.1 aLOUREIRO, M. E.  tFunctional & Integrative Genomicsgv. 13, n. 3, p. 379-389, 2013.