02480naa a2200373 a 450000100080000000500110000800800410001902400400006010000160010024501060011626000090022252014290023165000190166065300160167965300180169565300250171370000190173870000190175770000220177670000230179870000190182170000190184070000190185970000130187870000140189170000210190570000180192670000260194470000230197070000220199370000210201570000230203677300470205919818002017-09-20       2014    bl uuuu     u00u1 u    #d7 a10.1016/j.plantsci.2014.02.0032DOI1 aREIS, R. R.  aInduced over-expression of AtDREB2A CA improves drought tolerancein sugarcane.h[electronic resource]  c2014  atDrought is one of the most challenging agricultural issues limiting sustainable sugarcane production and,in some cases, yield losses caused by drought are nearly 50%. DREB proteins play vital regulatory rolesin abiotic stress responses in plants. The transcription factor DREB2A interacts with a cis-acting DREsequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in Arabidopsis thaliana. In the present study, we evaluated the effects of stress-inducibleover-expression of AtDREB2A CA on gene expression, leaf water potential (L), relative water content(RWC), sucrose content and gas exchanges of sugarcane plants submitted to a four-days water deficittreatment in a rhizotron-grown root system. The plants were also phenotyped by scanning the roots andmeasuring morphological parameters of the shoot. The stress-inducible expression of AtDREB2A CA intransgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress. Thetransgenic plants maintained higher RWC and Lover 4 days after withholding water and had higherphotosynthetic rates until the 3rd day of water-deficit. Induced expression of AtDREB2A CA in sugarcaneincreased sucrose levels and improved bud sprouting of the transgenic plants. Our results indicate thatinduced expression of AtDREB2A CA in sugarcane enhanced its drought tolerance without biomass penalty.  aabiotic stress  aAtDREB2A CA  aSaccharum spp  aTranscription factor1 aDIAS, B. B. A.1 aMARTINS, P. K.1 aMARTINS, M. T. B.1 aALEKCEVETCH, J. C.1 aJÚNIOR, A. C.1 aANDRADE, A. C.1 aRIBEIRO, A. P.1 aQIND, F.1 aMIZOI, J.1 aSHINOZAKI, K. I.1 aNAKASHIMA, K.1 aCARVALHO, J. de F. C.1 aSOUSA, C. A. F. de1 aNEPOMUCENO, A. L.1 aKOBAYASHI, A. K.1 aMOLINARI, H. B. C.  tPlant Sciencegv. 221-222, p. 59-68, 2014.