02182naa a2200289 a 450000100080000000500110000800800410001910000210006024501180008126000090019950001540020852011280036265000230149065000270151365000200154065000250156065300260158565300180161165300290162965300300165870000310168870000170171970000210173670000160175770000260177377300930179919817472017-07-06       2013    bl uuuu     u00u1 u    #d1 aFELIPE, R. T. A.  aReaction of sweet orange cultivars expressing the attacin A gene to 'Cadidatus Liberibacter asiaticus' infection.  c2013  aTítulo em português: Reação de cultivares de laranjeira doce que expressam o gene atacina A à infecção com 'Candidatus Liberibacterasiaticus'.  aThe objective of this work was to evaluate the reaction of four sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' (Las) infection, a bacterium associated to huanglongbing (HLB) disease. Transgenic sweet orange plants of Hamlin, Natal, Pêra, and Valência cultivars, as well as nontransgenic controls received inocula by grafting budwood sections of HLB‑infected branches. Disease progression was evaluated through observations of leaf symptoms and by polymerase chain reaction (PCR) analysis, eight months after inoculation. A completely randomized design was used, with four experiments (one for each cultivar) performed simultaneously. Bacteria title was estimated by quantitative PCR (qPCR). HLB symptoms and Las titers were present in nontransgenic and transgenic plants expressing the attacin A gene of the four sweet orange cultivars, eight months after bacteria inoculation. Five transgenic lines (transformation events) of 'Pêra' sweet orange expressing the attacin A gene have significantly lower Las titers in comparison with nontransgenic plants of this cultivar  aDisease resistance  aGenetic transformation  aCitrus Sinensis  aVariedade resistente  aAntibacterial peptide  aHuanglongbing  aPeptídeo antibacteriano  aTransformação genética1 aMOURÃO FILHO, F. de A. A.1 aLOPES, S. A.1 aMENDES, B. M. J.1 aBEHLING, M.1 aPEREIRA JUNIOR, E. V.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 48, n. 11, p. 1440-1448, nov. 2013.