02906naa a2200277 a 450000100080000000500110000800800410001902400620006010000200012224501550014226000090029752020390030665000200234565300110236565300220237665300080239865300310240665300100243770000170244770000230246470000230248770000250251070000240253570000180255977300510257719803542024-02-05       2013    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.theriogenology.2012.08.0152DOI1 aVIANA, J. H. M.  aOccurrence and characteristics of residual follicles formed after transvaginal ultrasound-guided follicle aspiration in cattle.h[electronic resource]  c2013  aUltrasound-guided transvaginal follicle aspiration is used to recover cumulus-oocyte complexes (for IVF) and to synchronize follicular wave emergence (ablation of dominant follicle). Although aspirated follicles are generally supposed to undergo immediate atresia, there are indications that they may remain active. The objective was to evaluate the occurrence and characteristics of residual follicles (RF) after transvaginal follicle aspiration in cattle. Ovarian follicular wave emergence was synchronized in Holstein cows (N = 13) in the presence (groups 1 and 3) or absence (groups 2 and 4) of norgestomet implants. The largest follicle was aspirated at a diameter of 8 mm (groups 1 and 2) or 12 mm (groups 3 and 4). Ovarian follicles were visualized (transrectal ultrasonography) every 12 h after wave emergence. Follicular fluid samples were collected from the largest follicle and from the ensuing RF and concentrations of estradiol and progesterone were determined. After aspiration, 73.2% (52/71) of the follicles refilled with fluid, and a new antrum was detected 12 to 24 h later. Norgestomet did not affect (P &gt 0.05) RF occurrence or diameter, but in RF from group 4, concentrations of estradiol decreased (-530.7 ± 133.9 ng/mL; P &lt 0.01) whereas progesterone increased (+429.6 ± 171.7 ng/mL; P &lt 0.05) relative to preaspiration. In RF, there were three steroidogenesis patterns: (1) high estradiol concentration and high estradiol:progesterone ratio (estradiol-active RF); (2) low estradiol, but high progesterone concentrations (luteinized RF); and (3) low estradiol and low progesterone concentrations (inactive RF). Estradiol-active RF were more likely (P &lt 0.05) from follicles with high estradiol concentrations (regardless of diameter). In conclusion, fluid-filled structures (RF) with variable steroid production patterns are frequently formed after ultrasound-guided follicle aspiration. The occurrence and features of these RF depended on the diameter and status of these follicles before aspiration.  asteroidogenesis  aBovine  aFollicle ablation  aOPU  aOvarian follicular atresia  aOvary1 aDOREA, M. D.1 aSIQUEIRA, L. G. B.1 aARASHIRO, E. K. N.1 aCAMARGO, L. S. de A.1 aFERNANDES, C. A. C.1 aPALHAO, M. P.  tTheriogenologygv. 79, n. 2, p. 267-273, 2013.