01919naa a2200253 a 450000100080000000500110000800800410001902400510006010000170011124501340012826000090026252011340027165000190140565000120142465000180143665300190145470000220147370000190149570000200151470000210153470000220155570000180157777300700159519792892024-02-05       2013    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.4238/2013.october.22.12DOI1 aNUNES, J. D.  aDNA elimination in embryogenic development of Pennisetum glaucum x Pennisetum purpureum (Poaceae) hybrids.h[electronic resource]  c2013  aInterspecific hybridization between Napier grass (Pennisetum purpureum), which is widely grown in Brazil for cattle forage, and pearl millet (Pennisetum glaucum) has been used as a breeding strategy for the development of improved cultivars. However, the hybrid between these two species is sterile due to its triploid condition (2n = 3x = 21 chromosomes), which hinders its use in crop breeding programs. It is known that genomic alterations result from the hybridization process. In order to measure the loss of DNA during embryo development, we used flow cytometry to estimate the nuclear DNA content of triploid and tetraploid embryos produced by interspecific hybridization between Napier grass and pearl millet. The triploid and tetraploid hybrids had a mean DNA content of 4.99-4.87 and 5.25-4.84 pg, at 10 and 30 days after pollination, respectively. The mean reduction in DNA content was higher in the tetraploid hybrids. The flow cytometry results revealed progressive genomic instability in these triploid and tetraploid hybrids, with this instability causing significant alterations in the DNA content of the hybrids.  aflow cytometry  agrasses  ahybridization  aElephant grass1 aAZEVEDO, A. L. S.1 aPEREIRA, A. V.1 aPAULA, C. M. P.1 aCAMPOS, J. M. S.1 aLEDO, F. J. da S.1 aSANTOS, V. B.  tGenetics and Molecular Researchgv. 12, n. 4, p. 4817-4826, 2013.