02256naa a2200277 a 450000100080000000500110000800800410001902400380006010000240009824501050012226000090022730000100023652014820024665000130172865000090174170000210175070000230177170000150179470000200180970000150182970000230184470000260186770000180189370000220191177300450193319782962022-04-07       2014    bl uuuu     u00u1 u    #d7 a10.1371/journal.pone.00864022DOI1 aMARCOLINO-GOMES, J.  aDiurnal oscillations of soybean circadian clock and drought responsive genes.h[electronic resource]  c2014  a13 p.  aRhythms produced by the endogenous circadian clock play a critical role in allowing plants to respond and adapt to the environment. While there is a well-established regulatory link between the circadian clock and responses to abiotic stress in model plants, little is known of the circadian system in crop species like soybean. This study examines how drought impacts diurnal oscillation of both drought responsive and circadian clock genes in soybean. Drought stress induced marked changes in gene expression of several circadian clock-like components, such as LCL1-, GmELF4- and PRR-like genes, which had reduced expression in stressed plants. The same conditions produced a phase advance of expression for the GmTOC1- like, GmLUX-like and GmPRR7-like genes. Similarly, the rhythmic expression pattern of the soybean drought-responsive genes DREB-, bZIP-, GOLS-, RAB18- and Remorin-like changed significantly after plant exposure to drought. In silico analysis of promoter regions of these genes revealed the presence of cis-elements associated both with stress and circadian clock regulation. Furthermore, some soybean genes with upstream ABRE elements were responsive to abscisic acid treatment. Our results indicate that some connection between the drought response and the circadian clock may exist in soybean since (i) drought stress affects gene expression of circadian clock components and (ii) several stress responsive genes display diurnal oscillation in soybeans.  aSoybeans  aSoja1 aRODRIGUES, F. A.1 aFUGANTI-PAGLIARINI1 aBENDIX, C.1 aNAKAYAMA, T. J.1 aCELAYA, B.1 aMOLINARI, H. B. C.1 aOLIVEIRA, M. C. N. de1 aHARMON, F. G.1 aNEPOMUCENO, A. L.  tPlos Onegv. 9, n. 1, e86402, Jan. 2014.