01033naa a2200265 a 450000100080000000500110000800800410001902400340006010000190009424502100011326000090032352001480033265300150048065300220049565300210051765300250053870000200056370000200058370000190060370000160062270000200063870000220065870000180068077300690069819754532023-05-02 2013 bl uuuu u00u1 u #d7 adoi: 10.1071/RDv26n1Ab312DOI1 aSARAIVA, N. Z. aProtocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kina sea ctivities in bovine cytoplasts obtained by chemical enucleation techniques.h[electronic resource] c2013 aChemical enucleation using microtubule-depolymerizing drugs is an attractive procedure to simplify the enucleation process in nuclear transfer. aMaturation aMitogen activated aPromoting factor aProtocol optmization1 aOLIVEIRA, C. S.1 aCOLLADO, M. del1 aLIMA, M. R. de1 aVANTINI, R.1 aMONTEIRO, F. M.1 aNICIURA, S. C. M.1 aGARCIA, J. M. tReproduction Fertility Devevelopmentgv. 26, n. 1, p. 130, 2013.