02444naa a2200373 a 450000100080000000500110000800800410001902400560006010000250011624501600014126000090030152012590031065000180156965000100158765000210159765000250161865000150164365000100165865300300166865300150169865300310171365300080174465300090175265300310176165300320179265300340182470000200185870000220187870000210190070000350192170000210195670000250197777300680200219735102015-03-18       2014    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1016/j.vetpar.2013.11.0062DOI1 aSANTOS, J. M. L. dos  aIdentification and quantification of benzimidazole resistance polymorphisms in Haemonchus contortus isolated in Northeastern Brazil.h[electronic resource]  c2014  aAbstract: Haemonchus contortus is the most prevalent nematode in Brazil. The objective of this study was to select 6 populations of H. contortus of known or suspected benzimidazole resistance status and characterize these using quantitative real-time polymerase chain reaction (qPCR) for single nucleotide polymorphisms (SNPs) F200Y, F167Y and E198A in the ?-tubulin isotype 1 gene. qPCR was performed using DNA from a pool of 10 adult male H. contortus from a single animal per farm. Faecal egg count reduction test (FECRT) and egg hatch test (EHT) were used to determine the resistance status. Samples were obtained from 6 farms located in 5 counties in the Ceará State: Tauá, Boa Viagem, Quixadá, Santa Quitéria and Solonópole. The inbred-susceptible-Edinburgh (ISE) isolate was used as reference for comparative purposes in the qPCR. Benzimidazole resistance was detected by FECRT on all farms with efficacy values ranging from 0 to 51%. EC50 values as determined by EHT were all above 1.49 ug/ml. High frequencies of the resistant SNPs F200Y and F167Y alleles were detected but no resistance was detected at SNP E198A. Our results suggest that the SNPs F167Y and F200Y are both important for benzimidazole resistance in the studied populations.  abenzimidazole  aSheep  aAnti-helmíntico  aHaemonchus contortus  aNematóide  aOvino  aGastrointestinal diseases  aHelmintose  aNematóide gastrintestinal  aPCR  aQPCR  aQuantitative real-time PCR  aResistance to anthelmintics  aResistência anti-helmíntica1 aMONTEIRO, J. P.1 aRIBEIRO, W. L. C.1 aMACEDO, I. T. F.1 aCAMURÇA-VASCONCELOS, A. L. F.1 aVIEIRA, L. da S.1 aBEVILÁQUA, C. M. L.  tVeterinary Parasitologygv. 199, n. 3/4, p. 160-164, Jan. 2014.