02321naa a2200301 a 450000100080000000500110000800800410001902400250006010000200008524501200010526000090022552014360023465000130167065000140168365000170169765300240171465300110173865300350174970000250178470000190180970000210182870000240184970000190187370000290189270000200192170000210194177300570196219695052020-01-09 2013 bl uuuu u00u1 u #d7 a10.1071/FP130962DOI1 aMORGANTE, C. V. aA survey of genes involved in Arachis stenosperma resistance to Meloidogyne arenaria race 1.h[electronic resource] c2013 aRoot-knot nematodes constitute a constraint for important crops, including peanut (Arachis hypogaea L.). Resistance to Meloidogyne arenaria has been identi fied in the peanut wild relative Arachis stenosperma Krapov. & W. C. Greg., in which the induction of feeding sites by the nematode was inhibited by an early hypersensitive response (HR). Here, the transcription expression pro fi les of 19 genes selected from Arachis species were analysed using quantitative reverse transcription?polymerase chain reaction (qRT-PCR), during the early phases of anA. stenosperma?M. arenaria interaction. Sixteen genes were signi ficantly differentially expressed in infected and non-infected roots, in at least one of the time points analysed: 3, 6, and 9 days after inoculation. These genes are involved in the HR and production of secondary metabolites related to pathogen defence. Seven genes encoding a resistance protein MG13, a helix-loop helix protein, an ubiquitin protein ligase, a patatin-like protein, a catalase, a DUF538 protein, and a resveratrol synthase, were differentially expressed in all time points analysed. Transcripts of two genes had their spatial and temporal distributions analysed by in situ hybridisation that validated qRT-PCR data. The identi fication of candidate resistance genes involved in wild peanut resistance to Meloidogyne can provide additional resources for peanut breeding and transgenic approaches. aAmendoim aNematoide aResistência aArachis stenosperma aPeanut aReação de hipersensibilidade1 aBRASILEIRO, A. C. M.1 aROBERTS, P. A.1 aGUIMARAES, L. A.1 aARAUJO, A. C. G. de1 aFONSECA, L. N.1 aBERTIOLI, S. C. de M. L.1 aBERTIOLI, D. J.1 aGUIMARAES, P. M. tFunctional Plant Biologygv. 41, p. 1298-1309, 2013.