02201naa a2200277 a 450000100080000000500110000800800410001902400550006010000160011524502120013126000090034352012830035265300210163565300150165665300150167170000200168670000160170670000190172270000260174170000150176770000200178270000190180270000160182170000230183777300630186019631492022-08-12       2013    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.exppara.2013.04.0062DOI1 aFAZA, A. P.  aA new approach to characterization of the resistance of populations of Rhipicephalus microplus (AcaribIxodidae) to organophosphate and pyrethroid in the State of Minas Gerais, Brazil.h[electronic resource]  c2013  aThe monitoring of resistance of cattle tick populations in Brazil to the chemical bases in use is largely limited to investigation of the phenotypic profile. There are few studies investigating the role played by the genotypic profile in acaricide resistance in the country. Therefore, the aim of the present study was to carry out molecular characterization and trace out the genetic profile of populations of Rhipicephalus microplus with respect to resistance to the organophosphate and pyrethroid chemical groups. For that purpose, larvae were genotyped belonging to 587 populations for pyrethroids and 306 for organophosphates, using the polymerase chain reaction technique. It was found that 75.49% and 97.44% of the larvae studied showed resistance to the organophosphates and pyrethroids, respectively. Among the populations resistant to pyrethroids, 91.9% were heterozygotes, showing that most of the resistant populations have only one allele responsible for resistance. Therefore, it is possible to conclude that the genotyped populations have high resistance to organophosphates, and even more so to pyrethroids. This information is fundamental for understanding the mechanisms of resistance of R. microplus to acaricides, to enable improvement of control techniques.  aCarrapato de boi  aGenotipico  aPiretroide1 aPINTO, I. S. B.1 aFONSECA, I.1 aANTUNES, G. R.1 aMONTEIRO, C. M. DE O.1 aDAEMON, E.1 aMUNIZ, M. de S.1 aMARTINS, M. F.1 aFURLONG, J.1 aPRATA, M. C. de A.  tExperimental Parasitologygv. 134, n. 4, p. 519-523, 2013.