03489naa a2200457 a 450000100080000000500110000800800410001902200140006010000210007424501140009526000090020950000190021852022430023765000120248065000130249265000240250565000110252965000250254065000190256565300270258470000190261170000200263070000230265070000220267370000180269570000220271370000200273570000180275570000280277370000190280170000280282070000190284870000270286770000250289470000170291970000150293670000180295170000190296970000210298877300220300919619972023-05-19       2012    bl uuuu     u00u1 u    #d  a2041-28511 aPASSOS, M. A. N.  aDevelopment of expressed sequence tag and EST-SSR marker resources for Musa acuminata.h[electronic resource]  c2012  a(Open Access).  aBackground and aims: Banana (Musa acuminata) is a crop contributing to global food security. Many varieties lack resistance to biotic stresses, due to sterility and narrow genetic background. The objective of this study was to develop an expressed sequence tag (EST) database of transcripts expressed during compatible and incompatible banana-Mycosphaerella fijiensis (Mf) interactions. Black leaf streak disease (BLSD), caused by Mf, is a destructive disease of banana. Microsatellite markers were developed as a resource for crop improvement. Methodology: cDNA libraries were constructed from in vitro-infected leafes from BLSD-resistant M. acuminata ssp. burmaniccoides Calcutta 4 (MAC4) and susceptible M. acuminata cv. Cavendish Grande Naine (MACV). Clones were 5´end Sanger sequenced, ESTs assembled with TGICL and unigenes annotated using BLAST, Blast2GO and InterProScan. Mreps was used to screen for simple sequence repeats (SSRs), with markers evaluated for polymorphism using twenty diploid (AA) M. acuminata accessions contrasting in resistance to Mycosphaerella leaf spot diseases. Principal results: A total of 9333 high quality ESTs were obtained for MAC4 and 3964 for MACV, which assembled into 3995 unigenes. Of these, 2592 displayed homology to genes encoding proteins with known or putative function, and 266 to genes encoding proteins with unknown function. Gene ontology (GO) classification identified 543 GO terms, 2300 unigenes were assigned to EuKaryotic Orthologous Groups (KOG) categories and 312 mapped to KEGG pathways. A total of 624 SSR loci were identified, with trinucleotide repeat motifs the most abundant in MAC4 (54.1%) and MACV Downloaded from http://aobpla.oxfordjournals.org/ at Empresa Brasileira de Pesquisa Agropecuária on July 12, 2013 4 (57.6%). Polymorphism across M. acuminata accessions was observed with 75 markers. Alleles per polymorphic locus ranged from 2 to 8, totaling 289. Polymorphism information content (PIC) ranged from 0.08 to 0.81. Conclusions: This EST collection offers a resource for studying functional genes, including transcripts expressed in banana-Mf interactions. Markers are applicable for genetic mapping, diversity characterization and marker assisted breeding.  aBananas  aBreeding  aGenetic improvement  aBanana  aMelhoramento vegetal  aMusa Acuminata  aMelhoramento genético1 aCRUZ, V. de O.1 aEMEDIATO, F. L.1 aTEIXEIRA, C. de C.1 aSOUZA JR. M. L T.1 aMATSUMOTO, T.1 aAZEVEDO, V. C. R.1 aFERREIRA, C. F.1 aAMORIM, E. P.1 aFIGUEIREDO, L. F. de A.1 aMARTINS, N. F.1 aCAVALCANTE, M. de J. B.1 aBAURENS, F.-C.1 aSILVA JUNIOR, O. B. da1 aPAPPAS JUNIOR, G. J.1 aPIGNOLET, L.1 aABADIE, C.1 aCIAMPI, A. Y.1 aPIFFANELLI, P.1 aMILLER, R. N. G.  tAOB Plants, 2012.