01865naa a2200241 a 450000100080000000500110000800800410001910000140006024501530007426000090022752011560023665000380139265000110143065000220144165000180146365300080148170000180148970000210150770000220152870000200155070000190157077300340158919615882023-05-19       2013    bl uuuu     u00u1 u    #d1 aMAFRA, V.  aCandidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype.h[electronic resource]  c2013  aCitrus huanglongbing (HLB) disease is caused by endogenous, phloem-restricted, Gram negative, uncultured bacteria named Candidatus Liberibacter africanus (CaLaf), Ca. L. asiaticus (CaLas), and Ca. L. americanus (CaLam), depending on the continent where the bacteria were first detected. The Asian citrus psyllid vector, Diaphorina citri, transmits CaLas and CaLam and both Liberibacter species are present in Brazil. Several studies of the transcriptional response of citrus plants manifesting HLB symptoms have been reported, but only for CaLas infection. This study evaluated the transcriptional reprogramming of a susceptible genotype of sweet orange challenged with CaLam, using a customized 385K microarray containing approximately 32,000 unigene transcripts. We analyzed global changes in gene expression of CaLam-infected leaves of sweet orange during the symptomatic stage of infection and compared the results with previously published microarray studies that used CaLas-infected plants. Twenty candidate genes were selected to validate the expression profiles in symptomatic and asymptomatic PCR-positive leaves infected with CaLas or CaLam.  aCandidatus Liberibacter africanus  aCitrus  aDoença de planta  aFruta citrica  aHLB1 aMARTINS, P. K1 aFRANCISCO, C. S.1 aRIBEIRO-ALVES, M.1 aASTUA, J. de F.1 aMACHADO, M. A.  tBMC Genomics, p.14-247, 2013.