04249naa a2200985 a 450000100080000000500110000800800410001902200140006002400280007410000180010224500980012026000090021852015650022765000110179265000110180370000210181470000250183570000200186070000240188070000130190470000230191770000200194070000170196070000230197770000220200070000230202270000200204570000180206570000190208370000230210270000230212570000230214870000240217170000250219570000170222070000160223770000220225370000230227570000180229870000190231670000300233570000230236570000210238870000190240970000120242870000290244070000220246970000180249170000220250970000220253170000170255370000220257070000210259270000180261370000170263170000220264870000160267070000230268670000230270970000220273270000220275470000240277670000210280070000160282170000200283770000160285770000180287370000180289170000170290970000180292670000180294470000170296270000170297970000180299670000250301470000200303970000140305970000220307370000210309570000210311670000170313770000190315470000290317377300610320219602152015-04-15       2013    bl uuuu     u00u1 u    #d  a1362-49627 a10.1093/nar/gkt4842DOI1 aMARINOTTI, O.  aThe Genome of Anopheles darlingi, the main neotropical malaria vector.h[electronic resource]  c2013  aAnopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors 100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vector?human and vector?parasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index. php/anopheles-darlingi.  aGenome  aGenoma1 aCERQUEIRA, G. C.1 aALMEIDA, L. G. P. de1 aFERRO, M. I. T.1 aLORETO, E. L. da S.1 aZAHA, A.1 aTEIXEIRA, S. M. R.1 aWESPISER, A. R.1 aSILVA, A. A.1 aSCHLINDWEIN, A. D.1 aPACHECO, A. C. L.1 aSILVA, A. L. da C.1 aGRAVELEY, B. R.1 aWALENZ, B. P.1 aLIMA, B. de A.1 aRIBEIRAO, C. A. G.1 aNUNES-SILVA, C. G.1 aCARVALHO, C. R. de1 aSOARES, C. M. de A.1 aMENEZES, C. B. A. de1 aMATIOLLI, C.1 aCAFFREY, D.1 aARAÚJO, D. A. M.1 aOLIVEIRA, D. M. de1 aGOLENBOCK, D.1 aGRISARD, E. C.1 aFANTINATTI-GARBOGGINI, F.1 aCARVALHO, F. M. de1 aBARCELLOS, F. G.1 aPROSDOCIMI, F.1 aMAY, G.1 aAZEVEDO JUNIOR, G. M. de1 aGUIMARÃES, G. M.1 aOLDMAN, G. H.1 aPADILHA, I. Q. M.1 aBATISTA, J. da S.1 aFERRO, J. A.1 aRIBEIRO, J. M. C.1 aFIETTO, J. L. R.1 aDABBAS, K. M.1 aCERDEIRA, L.1 aAGNEZ-LIMA, L. F.1 aBROCCHI, M.1 aCARVALHO, M. O. de1 aTEIXEIRA, M. de M.1 aMAIA, M. de M. D.1 aGOLDMAN, M. H. S.1 aSCHNEIDER, M. P. C.1 aFELIPE, M. S. S.1 aHUNGRIA, M.1 aNICOLÁS, M. F.1 aPEREIRA, M.1 aMONTES, A. M.1 aCANTAO, M. E.1 aVINCENTZ, M.1 aRAFAEL, M. S.1 aSILVERMAN, N.1 aSTOCO, P. H.1 aSOUZA, R. C.1 aVICENTINI, R.1 aGAZZINELLI, R. T. G.1 aNEVES, R. de O.1 aSILVA, R.1 aASTOLFI-FILHO, S.1 aMACIEL, T. E. F.1 aÜRMÉNYI, T. P.1 aTADEI, W. P.1 aCAMARGO, E. P.1 aVASCONCELOS, A. T. R. de  tNucleic Acid Researchgv. 41, n. 15, p. 7387-7400, 2013.