02078naa a2200289 a 450000100080000000500110000800800410001902400570006010000160011724500980013326000090023152012590024065000130149965000130151265000160152565000160154165300180155765300090157570000160158470000210160070000210162170000190164270000180166170000190167970000180169877300720171619431402022-09-05       2012    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1590/S0102-695X20120050000132DOI1 aPANDELO, D.  aOil production at different stages of leaf development in Lippia alba.h[electronic resource]  c2012  aThe aim of this work was to analyze terpene oil production and terpene synthases (TPS) gene expression from leaves at different developmental stages of different chemotypes of Lippia alba (Mill.) N.E. Br. ex Britton & P. Wilson, Verbenaceae. Hydro-distilled essential oil were used for chemical analysis and gene expression of three monoterpene synthase genes called LaTPS12, LaTPS23 and LaTPS25 were used for analyses of gene expression associated to oil production. The putative genes were associated to TPS-b gene class. Semi-quantitative PCR and quantitative PCR (qPCR) analysis were used to investigate the expression profile of those three putative genes in different leaf stages and different chemotypes. Additionally, total oil production and gene expression of putative TPS genes cloned from L. alba chemotype linalool were evaluated at different stages of leaf development. The expression level of those three genes was higher when the highest oil production was observed, mainly in young leaves at the fourth nodal segment for all evaluated chemotypes. Total oil production was higher at leaves that had unopened trichomes. We also observed that the 1mM of MeJA treatment increased the gene expression in all chemotypes after 24 h elicitation.  alimonene  alinalool  aLippia Alba  aVerbenaceae  aEssential oil  aQPCR1 aMELO, T. D.1 aSINGULANI, J. L.1 aGUEDES, F. A. F.1 aMACHADO, M. A.1 aCOELHO, C. M.1 aVICCINI, L. F.1 aSANTOS, M. O.  tRevista Brasileira de Farmacognosiagv. 22, n. 3, p. 497-501, 2012.