02200naa a2200253 a 450000100080000000500110000800800410001902400530006010000220011324501740013526000090030952014010031865000170171965000120173665000230174865000250177165300090179670000170180570000190182270000190184170000190186070000220187977300450190119422352022-08-09 2012 bl uuuu u00u1 u #d7 ahttps://doi.org/10.2135/cropsci2011.09.04802DOI1 aAZEVEDO, A. L. S. aCross species amplification of Penissetum glaucum microsatellite markers in penissetum purpureum and genetic diversity of Napier grass accessions.h[electronic resource] c2012 aNapier grass (Pennisetum purpureum Schum.) is an important forage crop in tropical areas although little is known about its genome information, and few molecular markers have been developed for this species. This work aimed to check the viability of cross-species amplifi cation of microsatellite markers between pearl millet (Pennisetum glaucum) and Napier grass and to evaluate the genetic diversity among Napier grass germplasm accessions. Fifty-four microsatellite markers previously described in pearl millet were tested against Napier grass, and 30 markers (55.5%) showed successful cross-amplifi cation. From them, 18 microsatellite markers were selected to study the genetic diversity in the Embrapa Active Germplasm Bank of Napier Grass (Embrapa-BAGCE). A total of 180 alleles were identifi ed by these selected microsatellite markers in 107 Napier grass accessions and four pearl millet samples. The average similarity coeffi cient (Dice) calculated among the Embrapa-BAGCE accessions was 0.651, ranging from 0.254 to 1.0. Some accessions showed similarity coeffi cients equal to one, indicating that they have common progenitors or that they might be the same accessions with different denomination. To our knowledge, this work is the fi rst to describe microsatellite markers in Napier grass and represents a signifi cant advance regarding the use of molecular markers in this specieS aCapim Napier aPainço aPennisetum Glaucum aVariação Genética aAFLP1 aCOSTA, P. P.1 aMACHADO, J. C.1 aMACHADO, M. A.1 aPEREIRA, A. V.1 aLEDO, F. J. da S. tCrop Sciencegv. 52, p. 1776-1785, 2012.