02223naa a2200277 a 450000100080000000500110000800800410001910000170006024501430007726000090022052013290022965000290155865000250158765000140161265000290162665000140165565000100166965000220167965300320170170000170173370000170175070000190176770000300178670000240181677301050184019386412012-11-12       2010    bl uuuu     u00u1 u    #d1 aBENEDUZI, A.  aPaenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Triticum aestivum.h[electronic resource]  c2010  aA bacterial strain designated SBR5T was isolated from the rhizosphere of Triticum aestivum. A phylogenetic analysis based on the 16S rRNA gene sequence placed the isolate within the genus Paenibacillus, being most closely related to Paenibacillus graminis RSA19T (98.1% similarity). The isolate was a Gram-reaction-variable, motile, facultatively anaerobic bacterium, with spores in a terminal position in cells. Starch was utilized and dihydroxyacetone and catalase were produced. Strain SBR5T displayed plant-growth-promoting rhizobacteria characteristics: the ability to fix nitrogen and to produce siderophores and indole-3-acetic acid. The DNA G+C content was 55.1 mol%. Chemotaxonomic analysis of the isolated strain revealed that MK-7 was the predominant menaquinone, while the major fatty acid was anteiso-C15:0. DNA?DNA hybridization values between strain SBR5T and P. graminis RSA19T, Paenibacillus odorifer TOD45T and Paenibacillus borealis KK19T were 43, 35 and 28%, respectively. These DNA relatedness data and the results of phylogenetic and phenotypic analyses showed that strain SBR5T should be considered as the nitrogen-fixing type strain of a novel species of the genus Paenibacillus, for which the name Paenibacillus riograndensis sp. nov. is proposed. The type strain is SBR5T (=CCGB 1313T =CECT 7330T).  aNitrogen-fixing bacteria  aRhizosphere bacteria  aBactéria  aFixação de nitrogênio  aRizosfera  aTrigo  aTriticum Aestivum  aPaenibacillus riograndensis1 aCOSTA, P. B.1 aPARMA, M. M.1 aMELO, I. S. de1 aBODANESE-ZANETTINI, M. H.1 aPASSAGLIA, L. M. P.  tInternational Journal of Systematic and Evolutionary Microbiology, Readinggv. 60, p. 128-133, 2010.