01566naa a2200217 a 450000100080000000500110000800800410001902200140006010000240007424501520009826000090025052009260025965000180118565000190120365300190122270000170124170000170125870000200127570000180129577300350131319350732023-05-19       2011    bl uuuu     u00u1 u    #d  a0567-75721 aRODRIGUEZ, M. A. D.  aA Greenhouse Bioassay for the Fusarium oxysporum f. sp. cubense x ?Grand Naine? (Musa, AAA, Cavendish Subgroup) Interaction.h[electronic resource]  c2011  aSeveral disease resistance screening protocols for Fusarium wilt of banana (causal agent Fusarium oxysporum f. sp. cubense - Foc) under greenhouse conditions have been reported. Here, we report a standardised rapid and reliable greenhouse bioassay for this pathosystem. This is indispensable for banana phenotyping, particularly since the occurrence of tropical race 4 (TR4), which is a significant threat for the global Cavendish-based banana export industry. Using a double-pot system, hardened 3-months-old tissue-culture plants of ?Grand Naine? (AAA, Cavendish subgroup) were individually inoculated with three TR4 isolates and one race 1 isolate with known pathogenicity on ?Silk? (AAB) bananas. All TR4 isolates caused similar symptoms and no differences regarding incubation period or severity were observed. In addition, all TR4 isolates were successfully recovered from the symptomatic rhizomes on Komada medium.  aFusarium wilt  atissue culture  aPanama disease1 aWAALWIJK, C.1 aPAIVA, L. V.1 aSOUZA JR. M. T.1 aG. H. J. KEMA  tActa Horticulturae, 897, 2011.