02269naa a2200277 a 450000100080000000500110000800800410001910000170006024500940007726000090017152015150018065000180169565300130171365300190172665300180174565300220176365300170178565300130180270000210181570000200183670000190185670000190187570000130189470000170190777300670192419333992012-09-11       2012    bl uuuu     u00u1 u    #d1 aRIGONATO, J.  aCyanobacterial diversity in the phyllosphere of a mangrove forest.h[electronic resource]  c2012  aThe cyanobacterial community colonizing phyllosphere in a well-preserved Brazilian mangrove ecosystem was assessed using cultivation-independent molecular approaches. Leaves of trees that occupy this environment (Rhizophora mangle, Avicennia schaueriana and Laguncularia racemosa) were collected along a transect beginning at the margin of the bay and extending upland. The results demonstrated that the phyllosphere of R. mangle and L. racemosa har- bor similar assemblages of cyanobacteria at each point along the transect. A. schaueriana, found only in the coastal portions of the transect, was colonized by assemblages with lower richness than the other trees. However, the results indicated that spatial location was a stronger driver of cyanobacterial commu- nity composition than plant species. Distinct cyanobacterial communities were observed at each location along the coast-to-upland transect. Clone library analysis allowed identification of 19 genera of cyanobacteria and demonstrated the presence of several uncultivated taxa. A predominance of sequences affili- ated with the orders Nostocales and Oscillatoriales was observed, with a remark- able number of sequences similar to genera Symphyonemopsis/Brasilonema (order Nostocales). The results demonstrated that phyllosphere cyanobacteria in this mangrove forest ecosystem are influenced by environmental conditions as the primary driver at the ecosystem scale, with tree species exerting some effect on community structure at the local scale.  aCyanobacteria  a16S rRNA  aCianobactéria  aClone library  aGenetic diversity  aLeaf surface  aPCR-DGGE1 aALVARENGA, D. O.1 aANDREOTE, F. D.1 aDIAS, A. C. F.1 aMELO, I. S. de1 aKENT, A.1 aFIORE, M. F.  tFEMS Microbiology Ecology, Amsterdamgv. 80, p. 312-322, 2012.