01756naa a2200217 a 450000100080000000500110000800800410001910000250006024501820008526000090026752009930027665300260126965300230129570000240131870000250134270000160136770000300138370000230141370000180143677300840145419213062012-03-31 2011 bl uuuu u00u1 u #d1 aANDRADE, A. R. O. de aFirst report of natural infection of phlebotomines for Leishmania (Leishmania) chagasi captured in Ponta Porã, on the border between Brazil and Paraguay.h[electronic resource] c2011 aObjective: Visceral leishmaniasis has been reported in all Brazilian regions and, do Sul State, the occurrence of cases has increased significantly. The objective of itnh iMs asttou dGyr owssaos btoo riddeenritnifgy Pthaera ngautauyr.a lM ineftehcotidosn: oBf yp hulseinbgo tloimghint eCsD bCy aLnedis Shhmaannnioan i ntr Paopns,t a1 8P5o rsãa,n ad fBlyra fzeimliaanle cso wunertye captured, dissected and arranged in 107 pools subjected to DNA extraction and amplification by Polymerase chain reaction. Results: From the samples subjected to amplification, the fragment of 1a2 f0e mbpa lceh sapreacciteesri sotfi cE voaf nLderioshmmyaian icao rstpel ewzazisi observed in one of the groups, which is composed by rate calculated for (Brèthes, 1923). Conclusions: The minimum infection of natural infection Pboyn Ltae iPshomrãa wniaas c0h.5a4g%a,s ia pnods eth ae soecrciouurrse cnocnec oefr nfl faogre tlhlaet etsra annsdm itshsei ocno noff ivrmisacteiroanl leishmaniasis in the region. aLeishmaniose visceral aMato Grosso do Sul1 aDORVAL, M. E. M. C.1 aANDRADE. S. M. O. de1 aMARQUES, A.1 aLIMA JÚNIOR, M. S. da C.1 aSILVA, B. A. K. da1 aANDREOTTI, R. tAsian Pacific Journal of Tropical Diseasegv.1, p. 253-336, Dec. 2011. Issue 4.